PMID- 9339686 OWN - NLM STAT- MEDLINE DA - 19971114 DCOM- 19971114 LR - 20061115 PUBM- Print IS - 0028-3878 (Print) VI - 49 IP - 4 DP - 1997 Oct TI - Higher neonatal cerebral blood flow correlates with worse childhood neurologic outcome. PG - 1035-41 AB - Cerebral blood flow (CBF) in newborn infants is often below levels necessary to sustain brain viability in adults. Controversy exists regarding the effects of such low CBF on subsequent neurologic function. We determined the current childhood neurologic status and IQ in 26 subjects who had measurements of CBF performed with PET in the neonatal period between 1983 and 1989 as part of a study of hypoxic-ischemic encephalopathy. Follow-up information at ages 4 to 12 years was obtained on all 26 subjects. Ten subjects had died. All 16 survivors underwent clinical neurologic evaluation, and 14 also underwent intelligence testing. Eight had abnormal clinical neurologic evaluations; eight were normal. The mean neonatal CBF in those with abnormal childhood neurologic outcome was significantly higher than in those with normal childhood neurologic outcome (35.64 +/- 11.80 versus 18.26 +/- 8.62 mL 100 g(-1) min(-1), t = 3.36, p = 0.005). A significant negative correlation between neonatal CBF and childhood IQ was demonstrated (Spearman rank correlation r = -0.675, p = 0.008). Higher CBF was associated with lower IQ. The higher CBF in subjects with worse neurologic and intellectual outcome may reflect greater loss of cerebrovascular autoregulation or other vascular regulatory mechanisms due to more severe brain damage. AD - Department of Pediatrics, Washington University School of Medicine, St. Louis Children's Hospital, MO 63110, USA. FAU - Rosenbaum, J L AU - Rosenbaum JL FAU - Almli, C R AU - Almli CR FAU - Yundt, K D AU - Yundt KD FAU - Altman, D I AU - Altman DI FAU - Powers, W J AU - Powers WJ LA - eng GR - NS06833/NS/NINDS GR - NS32568/NS/NINDS PT - Journal Article PT - Research Support, Non-U.S. Gov't PT - Research Support, U.S. Gov't, P.H.S. PL - UNITED STATES TA - Neurology JT - Neurology JID - 0401060 SB - AIM SB - IM MH - Cerebrovascular Circulation/*physiology MH - *Child Development MH - Follow-Up Studies MH - Humans MH - Infant, Newborn MH - Intelligence MH - *Nervous System Physiology MH - Neurologic Examination MH - Tomography, Emission-Computed EDAT- 1997/10/27 MHDA- 1997/10/27 00:01 PST - ppublish SO - Neurology. 1997 Oct;49(4):1035-41. PMID- 9357896 OWN - NLM STAT- MEDLINE DA - 19971120 DCOM- 19971120 LR - 20061115 PUBM- Print IS - 0003-3022 (Print) VI - 87 IP - 4 DP - 1997 Oct TI - Bupivacaine inhibition of L-type calcium current in ventricular cardiomyocytes of hamster. PG - 926-34 AB - BACKGROUND: The local anesthetic bupivacaine is cardiotoxic when accidentally injected into the circulation. Such cardiotoxicity might involve an inhibition of cardiac L-type Ca2+ current (ICa,L). This study was designed to define the mechanism of bupivacaine inhibition of ICa,L. METHODS: Cardiomyocytes were enzymatically dispersed from hamster ventricles. Certain voltage- and time-dependencies of ICa,L were recorded using the whole-cell patch clamp method in the presence and absence of different concentrations of bupivacaine. RESULTS: Bupivacaine, in a concentration-dependent manner (10-300 microM), tonically inhibited the peak amplitude of ICa,L. The inhibition was characterized by an increase in the time of recovery from inactivation and a negative-voltage shift of the steady-state inactivation curve. The inhibition was shown to be voltage-dependent, and the peak amplitude of ICa,L could not be restored to control levels by a wash from bupivacaine. CONCLUSIONS: The inhibition of ICa,L appears, in part, to result from bupivacaine predisposing L-type Ca channels to the inactivated state. Data from washout suggest that there may be two mechanisms of inhibition at work. Bupivacaine may bind with low affinity to the Ca channel and also affect an unidentified metabolic component that modulates Ca channel function. AD - Department of Anesthesiology, Winthrop-University Hospital, Mineola, New York, USA. krossner@epo.som.sunysb.edu FAU - Rossner, K L AU - Rossner KL FAU - Freese, K J AU - Freese KJ LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - UNITED STATES TA - Anesthesiology JT - Anesthesiology JID - 1300217 RN - 0 (Anesthetics, Local) RN - 0 (Calcium Channels) RN - 0 (Calcium Channels, L-Type) RN - 2180-92-9 (Bupivacaine) SB - AIM SB - IM MH - Anesthetics, Local/*pharmacology MH - Animals MH - Bupivacaine/*pharmacology MH - Calcium Channels/*drug effects MH - Calcium Channels, L-Type MH - Cricetinae MH - Dose-Response Relationship, Drug MH - Heart/*drug effects MH - Male EDAT- 1997/11/14 MHDA- 1997/11/14 00:01 PST - ppublish SO - Anesthesiology. 1997 Oct;87(4):926-34. PMID- 9298188 OWN - NLM STAT- MEDLINE DA - 19971117 DCOM- 19971117 LR - 20061115 PUBM- Print IS - 0003-6072 (Print) VI - 72 IP - 2 DP - 1997 Aug TI - Formation of formate and hydrogen, and flux of reducing equivalents and carbon in Ruminococcus flavefaciens FD-1. PG - 101-9 AB - A pathway for conversion of the metabolic intermediate phosphoenolpyruvate (PEP) and the formation of acetate, succinate, formate, and H2 in the anaerobic cellulolytic bacterium Ruminococcus flavefaciens FD-1 was constructed on the basis of enzyme activities detected in extracts of cells grown in cellulose- or cellobiose-limited continuous culture. PEP was converted to acetate and CO2 (via pyruvate kinase, pyruvate dehydrogenase, and acetate kinase) or carboxylated to form succinate (via PEP carboxykinase, malate dehydrogenase, fumarase, and fumarate reductase). Lactate was not formed even during rapid growth (batch culture, mu = 0.35/h). H2 was formed by a hydrogenase rather than by cleavage of formate, and 13C-NMR and 14C-exchange reaction data indicated that formate was produced by CO2 reduction, not by a cleavage of pyruvate. The distribution of PEP into the acetate and succinate pathways was not affected by changing extracellular pH and growth rates within the normal growth range. However, increasing growth rate from 0.017/h to 0.244/h resulted in a shift toward formate production, presumably at the expense of H2. This shift suggested that reducing equivalents could be balanced through formate or H2 production without affecting the yields of the major carbon-containing fermentation endproducts. AD - Department of Bacteriology, University of Wisconsin-Madison 53706, USA. FAU - Shi, Y AU - Shi Y FAU - Weimer, P J AU - Weimer PJ FAU - Ralph, J AU - Ralph J LA - eng PT - Journal Article PT - Research Support, U.S. Gov't, Non-P.H.S. PL - NETHERLANDS TA - Antonie Van Leeuwenhoek JT - Antonie van Leeuwenhoek JID - 0372625 RN - 0 (Acetates) RN - 0 (Bacterial Proteins) RN - 0 (Formates) RN - 110-15-6 (Succinic Acid) RN - 124-38-9 (Carbon Dioxide) RN - 127-17-3 (Pyruvic Acid) RN - 1333-74-0 (Hydrogen) RN - 16462-44-5 (Cellobiose) RN - 50-21-5 (Lactic Acid) RN - 73-89-2 (Phosphoenolpyruvate) RN - 9004-34-6 (Cellulose) RN - EC 1.18.99.1 (Hydrogenase) SB - IM MH - Acetates/*metabolism MH - Anaerobiosis MH - Bacterial Proteins/analysis/metabolism MH - Carbon Dioxide/metabolism MH - Cellobiose/metabolism MH - Cellulose/metabolism MH - Fermentation MH - Formates/*metabolism MH - Gram-Positive Cocci/enzymology/growth & development/*metabolism MH - Hydrogen/*metabolism MH - Hydrogen-Ion Concentration MH - Hydrogenase/metabolism MH - Lactic Acid/metabolism MH - Phosphoenolpyruvate/metabolism MH - Pyruvic Acid/metabolism MH - Succinic Acid/*metabolism EDAT- 1997/08/01 00:00 MHDA- 1997/09/23 00:01 PST - ppublish SO - Antonie Van Leeuwenhoek. 1997 Aug;72(2):101-9. PMID- 9263049 OWN - NLM STAT- MEDLINE DA - 19971027 DCOM- 19971027 LR - 20031114 PUBM- Print IS - 0021-8812 (Print) VI - 75 IP - 8 DP - 1997 Aug TI - Approximate confidence intervals for heritability from method R estimates. PG - 2041-6 AB - Method R estimates of heritability (h2) and associated confidence intervals (CI) were obtained from simulated data using a single trait, direct effects, full animal model, with 50% subsampling. Five hundred data sets were simulated for each of five levels of h2 (.10, .20, .30, .40, and .50) and two types of pedigree structure (random pedigree structure [N = 2,000] that varied over simulations, or the pedigree structure from a real data set [N = 2,644] that was constant for all simulations). The first 10, 20, and all 50 h2 estimates were used to obtain 80, 90, 95, and 99% CI for each data set. The variance of h2 estimates within data sets approximated the sampling variance of the h2 estimates. The Box-Cox transformation was used to normalize the distribution of estimates from each data set. Confidence intervals were computed on the transformed scale as CI = mu +/- (T x sigma), where mu and sigma = the mean and SD of the N transformed h2 estimates, respectively, and T = the critical value from the T distribution for a 1-alpha CI, with df = N-1. Upper and lower CI bounds were converted back to the original scale by reversing the transformation. The percentages of CI containing the true h2 value, pooled across all levels of h2, types of pedigree, and number of estimates used to obtain CI, for 80, 90, 95, and 99% CI were 81.14, 90.96, 95.27, and 98.76%, respectively. These results suggested that Method R h2 estimates can be used to obtain reliable CI. AD - Department of Statistics, Colorado State University, Fort Collins 80523, USA. FAU - Mallinckrodt, C H AU - Mallinckrodt CH FAU - Golden, B L AU - Golden BL FAU - Reverter, A AU - Reverter A LA - eng PT - Journal Article PL - UNITED STATES TA - J Anim Sci JT - Journal of animal science JID - 8003002 SB - IM MH - Analysis of Variance MH - Animals MH - Animals, Domestic/*genetics MH - Confidence Intervals MH - Female MH - *Genetics/statistics & numerical data MH - Male MH - *Models, Genetic MH - Phenotype MH - Time Factors EDAT- 1997/08/01 MHDA- 1997/08/01 00:01 PST - ppublish SO - J Anim Sci. 1997 Aug;75(8):2041-6. PMID- 9308130 OWN - NLM STAT- MEDLINE DA - 19971120 DCOM- 19971120 LR - 20060504 PUBM- Print IS - 0277-6715 (Print) VI - 16 IP - 18 DP - 1997 Sep 30 TI - rhDNase as an example of recurrent event analysis. PG - 2029-47 AB - We consider counting process methods for analysing time-to-event data with multiple or recurrent outcomes, using the models developed by Anderson and Gill, Wei, Lin and Weissfeld and Prentice, Williams and Peterson. We compare the methods, and show how to implement them using popular statistical software programs. By analysing three data sets, we illustrate the strengths and pitfalls of each method. The first example is simulated and involves the effect of a hidden covariate. The second is based on a trial of gamma interferon, and behaves remarkably like the first. The third and most interesting example involves both multiple events and discontinuous intervals at risk, and the three approaches give dissimilar answers. We recommend the AG and marginal models for the analysis of this type of data. AD - Mayo Clinic, Rochester, MI, USA. FAU - Therneau, T M AU - Therneau TM FAU - Hamilton, S A AU - Hamilton SA LA - eng PT - Journal Article PL - ENGLAND TA - Stat Med JT - Statistics in medicine JID - 8215016 RN - 0 (Expectorants) RN - 0 (Interferon-gamma, Recombinant) RN - 0 (Recombinant Proteins) RN - EC 3.1.21.1 (DNASE1 protein, human) RN - EC 3.1.21.1 (Deoxyribonuclease I) SB - IM MH - Adult MH - Analysis of Variance MH - Child MH - Cystic Fibrosis/*drug therapy MH - Data Collection/statistics & numerical data MH - Deoxyribonuclease I/*therapeutic use MH - Double-Blind Method MH - Expectorants/*therapeutic use MH - Granulomatous Disease, Chronic/*therapy MH - Humans MH - Interferon-gamma, Recombinant/*therapeutic use MH - *Mathematical Computing MH - *Models, Statistical MH - Randomized Controlled Trials/*statistics & numerical data MH - Recombinant Proteins/therapeutic use MH - Risk MH - *Software MH - *Survival Analysis MH - Treatment Outcome EDAT- 1997/10/06 17:35 MHDA- 2000/06/20 09:00 AID - 10.1002/(SICI)1097-0258(19970930)16:18<2029::AID-SIM637>3.0.CO;2-H [pii] PST - ppublish SO - Stat Med. 1997 Sep 30;16(18):2029-47. PMID- 9317033 OWN - NLM STAT- MEDLINE DA - 19971030 DCOM- 19971030 LR - 20061115 PUBM- Print IS - 0019-9567 (Print) VI - 65 IP - 10 DP - 1997 Oct TI - Involvement of p21racA, phosphoinositide 3-kinase, and vacuolar ATPase in phagocytosis of bacteria and erythrocytes by Entamoeba histolytica: suggestive evidence for coincidental evolution of amebic invasiveness. PG - 4243-9 AB - Trophozoites of Entamoeba histolytica, the protozoan parasite that causes amebic dysentery, phagocytose bacteria in the colonic lumen and erythrocytes (RBC) in host tissues. Because tissue invasion is an evolutionary dead end, it is likely that amebic pathogenicity is coincidentally selected, i.e., the same methods used to kill bacteria in the colonic lumen are used by parasites to damage host cells and cause disease. In support of this idea, the amebic lectin and pore-forming peptide are involved in binding and killing, respectively, bacteria and host epithelial cells. Here amebic phagocytosis of bacteria, RBC, and mucin-coated beads was disrupted by overexpression of E. histolytica p21(racA-V12), a ras-family protein involved in selection of sites of actin polymerization, which had been mutated to eliminate its GTPase activity. p21(racA-V12) transformants were also defective in capping and cytokinesis, while pinocytosis of fluorescent dextrans was not affected. Wortmannin, a fungal inhibitor of phosphoinositide 3-kinase, markedly inhibited phagocytosis of bacteria, RBC, and mucin-coated beads by wild-type amebae. In contrast to p21(racA-V12) overexpression, wortmannin abolished amebic pinocytosis of dextrans but had no inhibitory effects on capping. Inhibition of amebic vacuolar acidification by bafilomycin also decreased bacterial and RBC uptake. These results, which demonstrate similarities between mechanisms of phagocytosis of bacteria and RBC by amebae and macrophages, support the idea of coincidental selection of amebic genes encoding proteins that mediate destruction of host cells. AD - Department of Tropical Public Health, Harvard School of Public Health, Boston, Massachusetts 02115, USA. FAU - Ghosh, S K AU - Ghosh SK FAU - Samuelson, J AU - Samuelson J LA - eng GR - AI-33492/AI/NIAID PT - Journal Article PT - Research Support, U.S. Gov't, P.H.S. PL - UNITED STATES TA - Infect Immun JT - Infection and immunity JID - 0246127 RN - 0 (Androstadienes) RN - 0 (Anti-Bacterial Agents) RN - 0 (Cysteine Proteinase Inhibitors) RN - 0 (Macrolides) RN - 12125-02-9 (Ammonium Chloride) RN - 17090-79-8 (Monensin) RN - 19545-26-7 (wortmannin) RN - 61-90-5 (Leucine) RN - 66701-25-5 (E 64) RN - EC 2.7.1.137 (1-Phosphatidylinositol 3-Kinase) RN - EC 3.6.1.- (GTP-Binding Proteins) RN - EC 3.6.5.2 (rac GTP-Binding Proteins) SB - IM MH - 1-Phosphatidylinositol 3-Kinase/genetics/*metabolism MH - Ammonium Chloride/pharmacology MH - Androstadienes/pharmacology MH - Animals MH - Anti-Bacterial Agents/pharmacology MH - Bacteria MH - Cysteine Proteinase Inhibitors/pharmacology MH - Entamoeba histolytica/cytology/genetics/*pathogenicity MH - Erythrocytes MH - Evolution MH - GTP-Binding Proteins/genetics/*metabolism MH - Hydrogen-Ion Concentration MH - Immunologic Capping MH - Leucine/analogs & derivatives/pharmacology MH - Macrolides MH - Macrophages/physiology MH - Models, Biological MH - Monensin/pharmacology MH - Mutation MH - Phagocytosis/*physiology MH - Pinocytosis MH - Transformation, Genetic MH - Vacuoles/physiology MH - rac GTP-Binding Proteins EDAT- 1997/10/08 MHDA- 1997/10/08 00:01 PST - ppublish SO - Infect Immun. 1997 Oct;65(10):4243-9. PMID- 9331038 OWN - NLM STAT- MEDLINE DA - 19971106 DCOM- 19971106 LR - 20041117 PUBM- Print IS - 0301-620X (Print) VI - 79 IP - 5 DP - 1997 Sep TI - Intra-articular local anaesthesia for pain after hip arthroplasty. PG - 796-800 AB - We investigated 15 patients with painful hip arthroplasties using intra-articular injection of bupivicaine. Fourteen had pain relief and 13 of them were subsequently found to have loosening of one or both components. The relief of pain after total hip arthroplasty by intra-articular injection of bupivicaine indicates that a satisfactory result is probable after revision surgery with refixation of the components. AD - Princess Elizabeth Orthopaedic Hospital, Exeter, UK. FAU - Crawford, R W AU - Crawford RW FAU - Ellis, A M AU - Ellis AM FAU - Gie, G A AU - Gie GA FAU - Ling, R S AU - Ling RS LA - eng PT - Journal Article PL - ENGLAND TA - J Bone Joint Surg Br JT - The Journal of bone and joint surgery. British volume JID - 0375355 RN - 0 (Anesthetics, Local) RN - 2180-92-9 (Bupivacaine) SB - AIM SB - IM MH - Aged MH - Aged, 80 and over MH - Anesthetics, Local/*therapeutic use MH - Arthrography MH - Bupivacaine/*therapeutic use MH - Female MH - Follow-Up Studies MH - Hip Prosthesis/*adverse effects MH - Humans MH - Injections, Intra-Articular MH - Male MH - Middle Aged MH - Pain Measurement MH - Pain, Postoperative/*drug therapy/*etiology/radiography MH - Prosthesis Failure EDAT- 1997/10/23 MHDA- 1997/10/23 00:01 PST - ppublish SO - J Bone Joint Surg Br. 1997 Sep;79(5):796-800. PMID- 9382160 OWN - NLM STAT- MEDLINE DA - 19971110 DCOM- 19971110 LR - 20070214 PUBM- Print IS - 0250-8095 (Print) VI - 17 IP - 5 DP - 1997 TI - Determinants of type of initial hemodialysis vascular access. PG - 425-7 AB - Vascular access thrombosis is more common with polytetrafluoroethylene (PTFE) grafts than with native arteriovenous fistulae (AVF). Recent studies report an unexplained excess vascular access morbidity in women on hemodialysis. We studied 92 consecutive end-stage renal disease (ESRD) patients receiving their first permanent hemodialysis vascular access at initiation of hemodialysis to identify variables that determine assignment of either a PTFE graft or a native AVF. Independent variables included: age, gender, race, etiology of ESRD, and whether or not access surgery was electively planned before need for dialytic therapy. The 51 women and 41 men included 65 blacks, 13 Hispanics, 11 whites, and 3 Orientals aged 50 +/- (SD) 16 years. Of the 92 subjects, 54 (59%) received an AVF, while 38 (41%) received a PTFE graft. 36 (94%) of 38 PTFE grafts were placed in the upper arm as compared with 9 (17%) of 54 AVF (p = 0.0001). Also, 45 (83%) of 54 AVF were placed in the forearm as compared with only 2 (6%) of 38 PTFE grafts (p = 0.0001). Women were more likely to receive a PTFE graft - 28 (55%) of 51 - than men - 10 (24%) of 41 (p = 0.003). By contrast, men were more likely to get an AVF - 31 (76%) of 41 - than women - 23 (45 %) of 51 (p = 0.003). The log linear analysis confirmed that this finding was significant (p = 0.0018) for the coefficient of interaction between gender and type of vascular access. No other independent variable had a significant relationship with type of vascular access. We conclude that women with ESRD are more likely to receive a PTFE graft for hemodialysis, while men are more likely to get an AVF. These findings may explain, in part, the reported excess vascular access morbidity in women on hemodialysis. AD - Department of Medicine, Scientific and Academic Computing Center, State University of New York Health Science Center, Brooklyn 11203, USA. FAU - Ifudu, O AU - Ifudu O FAU - Macey, L J AU - Macey LJ FAU - Homel, P AU - Homel P FAU - Hyppolite, J C AU - Hyppolite JC FAU - Hong, J AU - Hong J FAU - Sumrani, N AU - Sumrani N FAU - Distant, D AU - Distant D FAU - Sommer, B G AU - Sommer BG FAU - Friedman, E A AU - Friedman EA LA - eng PT - Comparative Study PT - Journal Article PL - SWITZERLAND TA - Am J Nephrol JT - American journal of nephrology JID - 8109361 RN - 9002-84-0 (Polytetrafluoroethylene) SB - IM MH - Adult MH - Aged MH - Aged, 80 and over MH - *Arteriovenous Shunt, Surgical/adverse effects MH - *Catheters, Indwelling/adverse effects MH - Decision Making MH - Female MH - Graft Occlusion, Vascular/etiology MH - Humans MH - Kidney Failure, Chronic/therapy MH - Male MH - Middle Aged MH - Polytetrafluoroethylene/adverse effects MH - Prospective Studies MH - Renal Dialysis/*methods MH - Sex Factors MH - Thrombosis/epidemiology/etiology EDAT- 1997/01/01 00:00 MHDA- 1997/10/27 00:01 PST - ppublish SO - Am J Nephrol. 1997;17(5):425-7. PMID- 9301125 OWN - NLM STAT- MEDLINE DA - 19971124 DCOM- 19971124 LR - 20061115 PUBM- Print IS - 0916-8451 (Print) VI - 61 IP - 8 DP - 1997 Aug TI - Pradimicin, a mannose-binding antibiotic, induced carbohydrate-mediated apoptosis in U937 cells. PG - 1408-10 AB - Pradimicin (PRM), a mannose-binding antifungal antibiotic, recognizes a D-mannoside in the presence of calcium. We demonstrated that BMY-28864, a semi-synthetic analog of PRM, induced apoptosis in U937 cells which had been incubated with 1-deoxymannojirimycin (DMJ). Characteristic morphological changes such as formation of apoptotic bodies and DNA fragmentation were observed in apoptotic cells. AD - Toyama Prefectural University, Biotechnology Research Center, Japan. oki@pu-toyama.ac.jp FAU - Oki, T AU - Oki T FAU - Yamazaki, Y AU - Yamazaki Y FAU - Furumai, T AU - Furumai T FAU - Igarashi, Y AU - Igarashi Y LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - JAPAN TA - Biosci Biotechnol Biochem JT - Bioscience, biotechnology, and biochemistry JID - 9205717 RN - 0 (Anti-Bacterial Agents) RN - 0 (Antibiotics, Antineoplastic) RN - 0 (Antigens, Surface) RN - 0 (Carbohydrates) RN - 0 (DNA, Neoplasm) RN - 0 (Oligosaccharides) RN - 19130-96-2 (1-Deoxynojirimycin) RN - 31103-86-3 (Mannose) SB - B MH - 1-Deoxynojirimycin/pharmacology MH - Anti-Bacterial Agents/*pharmacology MH - Antibiotics, Antineoplastic/*pharmacology MH - Antigens, Surface/biosynthesis MH - Apoptosis/*drug effects MH - Carbohydrates/*physiology MH - Cell Line MH - DNA, Neoplasm/biosynthesis/chemistry MH - Electrophoresis, Agar Gel MH - Humans MH - Leukemia, Myeloid/metabolism MH - Mannose/metabolism MH - Oligosaccharides/biosynthesis EDAT- 1997/08/01 00:00 MHDA- 1997/09/25 00:01 PST - ppublish SO - Biosci Biotechnol Biochem. 1997 Aug;61(8):1408-10. PMID- 9378488 OWN - NLM STAT- MEDLINE DA - 19971107 DCOM- 19971107 LR - 20061115 PUBM- Print IS - 0019-2805 (Print) VI - 91 IP - 4 DP - 1997 Aug TI - TCR alpha beta+ CD4- CD8- T cells differentiate extrathymically in an lck-independent manner and participate in early response against Listeria monocytogenes infection through interferon-gamma production. PG - 511-9 AB - T-cell receptor (TCR) alpha beta+ CD4- CD8- (double-negative; DN) T cells appear in the peritoneal cavity at an early stage of intraperitoneal (i.p.) infection with the intracellular pathogen Listeria monocytogenes. In the present report, we analysed the developmental pathway and functions of the TCR alpha beta+ DN T cells using the L. monocytogenes infection system. The TCR alpha beta+ DN T cells appeared in the peritoneal cavity after L. monocytogenes i.p. infection in adult-thymectomized lethally irradiated bone marrow chimeras and p56lck-deficient mice. The results demonstrated that the TCR alpha beta+ DN T cells can develop extrathymically in a p56lck-independent manner. Reverse transcription-polymerase chain reaction (RT-PCR) analysis showed that the TCR alpha beta+ DN T cells expressed genes for interferon-gamma (IFN-gamma), the macrophage chemotactic factors MCP-1 and Eta-1, and granulocyte-macrophage colony-stimulating factor (GM-CSF) but lacked expression of genes for interleukin-2 (IL-2), IL-4 and IL-10. As expected from the RT-PCR analysis, the TCR alpha beta+ DN T cells produced IFN-gamma in response to anti-TCR beta monoclonal antibody (mAb), anti-CD3 mAb and L. monocytogenes-infected macrophages but IL-4 was undetectable after the stimulation. Furthermore, the intracellular cytokine staining analysis demonstrated that approximately half of the TCR alpha beta+ DN T cells detectable at the early stage of L. monocytogenes infection were IFN-gamma-producing cells. All of the results suggest that the TCR alpha beta+ DN T cells develop through a unique extrathymic p56lck-independent pathway and participate in early protection against bacterial infection through activation and accumulation of macrophages. AD - Department of Immunology, Kyushu University, Japan. FAU - Kadena, T AU - Kadena T FAU - Matsuzaki, G AU - Matsuzaki G FAU - Fujise, S AU - Fujise S FAU - Kishihara, K AU - Kishihara K FAU - Takimoto, H AU - Takimoto H FAU - Sasaki, M AU - Sasaki M FAU - Beppu, M AU - Beppu M FAU - Nakamura, S AU - Nakamura S FAU - Nomoto, K AU - Nomoto K LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - ENGLAND TA - Immunology JT - Immunology JID - 0374672 RN - 0 (Antigens, CD4) RN - 0 (Antigens, CD8) RN - 0 (Cytokines) RN - 0 (Receptors, Antigen, T-Cell, alpha-beta) RN - 82115-62-6 (Interferon Type II) RN - EC 2.7.1.37 (Lymphocyte Specific Protein Tyrosine Kinase p56(lck)) SB - IM MH - Animals MH - Antigens, CD4/analysis MH - Antigens, CD8/analysis MH - Ascitic Fluid/immunology MH - Cell Culture Techniques MH - Cell Differentiation/immunology MH - Cytokines/genetics/metabolism MH - Female MH - Gene Expression MH - Interferon Type II/*biosynthesis MH - Listeria Infections/*immunology MH - Lymphocyte Specific Protein Tyrosine Kinase p56(lck)/*deficiency/immunology MH - Mice MH - Mice, Inbred Strains MH - Mice, Knockout MH - Polymerase Chain Reaction MH - Receptors, Antigen, T-Cell, alpha-beta/*analysis MH - T-Lymphocyte Subsets/*immunology EDAT- 1997/08/01 00:00 MHDA- 1997/10/24 00:01 PST - ppublish SO - Immunology. 1997 Aug;91(4):511-9. PMID- 9298438 OWN - NLM STAT- MEDLINE DA - 19971024 DCOM- 19971024 LR - 20061115 PUBM- Print IS - 0160-7715 (Print) VI - 20 IP - 4 DP - 1997 Aug TI - Identification of Beck Depression Inventory items related to multiple sclerosis. PG - 407-14 AB - The percentage contribution of each item on the Beck Depression Inventory (BDI) to the total BDI score was compared across patients with multiple sclerosis (MS), patients diagnosed with major depressive disorder, and normal college students. We considered an item to be confounded by MS-related symptoms if its percentage contribution to the total BDI score was significantly greater in the MS group than the major depression and control groups. Items measuring work difficulty, fatigue, and concerns about health met this criterion. These items accounted for 34, 17, and 19% of the total BDI score in the MS, major depression, and control groups, respectively. Using the 18-item BDI (BDI-18) which resulted from excluding the 3 confounded items, MS patients found to be were more depressed than controls but less depressed than the major depression group. The identification of signs of depression not confounded with MS which could be substituted for confounded signs was also discussed. AD - UCSF/Mt. Zion MS Center 94115-1642, USA. FAU - Mohr, D C AU - Mohr DC FAU - Goodkin, D E AU - Goodkin DE FAU - Likosky, W AU - Likosky W FAU - Beutler, L AU - Beutler L FAU - Gatto, N AU - Gatto N FAU - Langan, M K AU - Langan MK LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - UNITED STATES TA - J Behav Med JT - Journal of behavioral medicine JID - 7807105 SB - IM MH - Adult MH - Depressive Disorder/diagnosis/*psychology MH - Female MH - Humans MH - Male MH - Middle Aged MH - Multiple Sclerosis/diagnosis/*psychology MH - Personality Inventory/*statistics & numerical data MH - Psychometrics MH - Reproducibility of Results MH - Sick Role EDAT- 1997/08/01 00:00 MHDA- 1997/09/23 00:01 PST - ppublish SO - J Behav Med. 1997 Aug;20(4):407-14. PMID- 9279722 OWN - NLM STAT- MEDLINE DA - 19971028 DCOM- 19971028 LR - 20061115 PUBM- Print IS - 0163-4453 (Print) VI - 35 IP - 1 DP - 1997 Jul TI - Early appearance of neutralizing antibodies after vaccination with an inactivated hepatitis A vaccine. PG - 37-40 AB - Sera from 30 subjects vaccinated with the Pasteur Merieux Serums & Vaccins (PM) inactivated hepatitis A vaccine, and from 30 subjects vaccinated with the Smithkline Beecham (SB) inactivated hepatitis A vaccine, were tested in two laboratories in order to provide comparative data on neutralizing activities of vaccine-induced antibodies. Sera were also evaluated by a modified radioimmunoassay (mRIA) and results were compared to neutralization assays results. Neutralizing antibody titres provided by the two laboratories correlated well (coefficient or correlation 0.42, P < 0.001). Neutralizing antibodies were detected after vaccination with both vaccines, and the kinetics of neutralizing antibody were the same with both vaccines. The titres gradually increased between the second week after the first dose and the post-booster dose (week 28). A strong booster effect of the booster vaccine dose on neutralizing titres was observed. Significantly higher neutralizing antibody titres with the PM vaccine were observed as early immune response on week 2 titres on both series of results. Vaccine-induced neutralizing antibody titres and vaccine-induced antibody mRIA titres correlated well (coefficient of correlation 0.82 and 0.72, respectively, P < 0.0001 in both cases). These results demonstrate early appearance of neutralizing antibody at high titre with the PM vaccine. AD - Hygiene-Institut der Universitat Tubingen, Abteilung fur Medizinische Virologie und Epidemiologie der Viruskrankheiten, Germany. FAU - Flehmig, B AU - Flehmig B FAU - Staedele, H AU - Staedele H FAU - Xueref, C AU - Xueref C FAU - Vidor, E AU - Vidor E FAU - Zuckerman, J AU - Zuckerman J FAU - Zuckerman, A AU - Zuckerman A LA - eng PT - Clinical Trial PT - Comparative Study PT - Controlled Clinical Trial PT - Journal Article PL - ENGLAND TA - J Infect JT - The Journal of infection JID - 7908424 RN - 0 (Antibodies, Viral) RN - 0 (Hepatitis A Vaccines) RN - 0 (Vaccines, Inactivated) RN - 0 (Viral Hepatitis Vaccines) SB - IM MH - Adolescent MH - Adult MH - Antibodies, Viral/*analysis MH - Female MH - Hepatitis A/prevention & control MH - Hepatitis A Vaccines MH - Hepatitis A Virus, Human/*immunology MH - Humans MH - Male MH - Neutralization Tests MH - Radioimmunoassay MH - Vaccines, Inactivated/administration & dosage MH - Viral Hepatitis Vaccines/*administration & dosage EDAT- 1997/07/01 MHDA- 1997/07/01 00:01 AID - S0163-4453(97)90929-4 [pii] PST - ppublish SO - J Infect. 1997 Jul;35(1):37-40. PMID- 9366534 OWN - NLM STAT- MEDLINE DA - 19971125 DCOM- 19971125 LR - 20041117 PUBM- Print IS - 0009-9260 (Print) VI - 52 IP - 10 DP - 1997 Oct TI - MRI and CT of metastatic hepatocellular carcinoma causing spinal cord compression. PG - 755-60 AB - We report the imaging features in five patients with metastatic hepatocellular carcinoma causing spinal cord compression, three of which were biopsy proven and two were in patients with known diagnosis of hepatocellular carcinoma. The radiographic, magnetic resonance imaging (MRI) and computed tomography (CT) features are highlighted. Although the occurrence of metastatic disease in hepatocellular carcinoma is exceedingly rare, it may be increasingly encountered as survival of patients is improved with advancing methods of therapy, both surgical and palliative. It often accompanies local recurrence, and invariably signals a grave prognosis with extremely short life expectancy. Unusually, two of the five patients in this series presented initially with skeletal metastases which led to the diagnosis of hepatocellular carcinoma. AD - Department of Diagnostic Radiology & Organ Imaging, Chinese University of Hong Kong, Hong Kong. FAU - Yang, W T AU - Yang WT FAU - Yeo, W AU - Yeo W FAU - Leung, S F AU - Leung SF FAU - Chan, Y L AU - Chan YL FAU - Johnson, P J AU - Johnson PJ FAU - Metreweli, C AU - Metreweli C LA - eng PT - Case Reports PT - Journal Article PL - ENGLAND TA - Clin Radiol JT - Clinical radiology JID - 1306016 SB - IM MH - Adult MH - Bone Neoplasms/radiography/*secondary MH - Carcinoma, Hepatocellular/complications/diagnosis/*secondary MH - Fatal Outcome MH - Female MH - Humans MH - Liver Neoplasms/*pathology MH - *Magnetic Resonance Imaging MH - Male MH - Middle Aged MH - Spinal Cord Compression/*etiology MH - Spinal Neoplasms/complications/secondary MH - *Tomography, X-Ray Computed EDAT- 1997/11/20 MHDA- 1997/11/20 00:01 PST - ppublish SO - Clin Radiol. 1997 Oct;52(10):755-60. PMID- 9293364 OWN - NLM STAT- MEDLINE DA - 19971030 DCOM- 19971030 LR - 20041117 PUBM- Print IS - 0967-2109 (Print) VI - 5 IP - 3 DP - 1997 Jun TI - Transected thoracic aorta: age-specific differences in incidence and possible reasons. PG - 291-4 AB - The objective of this study was to determine the incidence of aortic transaction in relation to age, and to examine possible reasons for the observed differences. Data from the North Carolina Medical Database over a 7-year period were examined for the total number of motor vehicle accident victims and for the subset with aortic rupture, based on age at presentation. Data were then divided into 10-year intervals and the differences analyzed using chi-square analysis. Differences among various age groups were statistically significant (P = 0.0001). The highest rate was in the 21-30-year-old age group and average incidence for all ages was 0.7%. High incidence of aortic transaction in the 21-30-year-old group may be due to an increase in high-risk behaviors in such persons, to an improved survival compared with other age ranges, or to an inherent susceptibility of the aorta at this stage of life. These data have important implications for the diagnosis and treatment of aortic transaction and should be taken into account when developing practice guidelines for its management. AD - Department of Thoracic and Cardiovascular Surgery, Carolinas Heart Institute, Carolinas Medical Center, North Carolina, USA. FAU - Tripp, H F AU - Tripp HF FAU - Robicsek, F AU - Robicsek F FAU - Thomason, M AU - Thomason M FAU - Thubrikar, M AU - Thubrikar M LA - eng PT - Journal Article PL - ENGLAND TA - Cardiovasc Surg JT - Cardiovascular surgery (London, England) JID - 9308765 SB - IM MH - Accidents, Traffic/mortality MH - Adolescent MH - Adult MH - Age Factors MH - Aged MH - Aged, 80 and over MH - Aorta, Thoracic/*injuries/surgery MH - Aortic Rupture/etiology/mortality/*surgery MH - Child MH - Child, Preschool MH - Cross-Sectional Studies MH - Female MH - Humans MH - Incidence MH - Infant MH - Male MH - Middle Aged MH - North Carolina/epidemiology MH - Registries/statistics & numerical data MH - Risk Factors MH - Sex Factors MH - Survival Analysis MH - Thoracic Injuries/etiology/mortality/*surgery MH - Wounds, Nonpenetrating/etiology/mortality/*surgery EDAT- 1997/06/01 00:00 MHDA- 1997/09/18 00:01 AID - S0967-2109(97)00019-7 [pii] PST - ppublish SO - Cardiovasc Surg. 1997 Jun;5(3):291-4. PMID- 9377289 OWN - NLM STAT- MEDLINE DA - 19971125 DCOM- 19971125 LR - 20061115 PUBM- Print IS - 0210-0010 (Print) VI - 25 IP - 145 DP - 1997 Sep TI - [Clinical variability of bilateral paramedian thalamic infarcts] PG - 1353-62 AB - INTRODUCTION: Thalamic infarcts in paramedian artery territory are seen fairly frequently owing to certain peculiarities of the vascularization of the thalamus. However, clinical diagnosis is usually difficult because of the many varieties and peculiarities of the symptomatology. MATERIAL AND METHODS: We present a review of twelve cases of bilateral paramedian infarcts of the thalamus seen in our Department of Neurology and in a private surgery. We analyze the symptoms and their relationship to the neuro-radiological findings. Finally we compare our observations with the descriptions published by other authors and seek and anatomo-functional relationship for each of the symptoms and signs observed. RESULTS: The usual clinical outline in our patients included disorders of consciousness, different types of oculomotor disorders and cerebellar symptoms, mainly of gait. Other less common findings were memory disorders and abnormal movements. In no case were there sensory changes and pyramidal signs were rare in the absence of significant extra-thalamic lesions. CONCLUSIONS: Our findings, although generally comparable to those described in the literature consulted, were somewhat different with regard to cerebellar symptoms and the absence of sensory and pyramidal signs. We also emphasize the marked differences seen between the individual patients in our series. A good knowledge of the possible clinical variants of these lesions is necessary for a correct initial diagnostic approach in the study of these patients. AD - Servicio de Neurologia, Hospital Ntra. Sra. de Aranzazu, San Sebastian, Guipuzcoa, Espana. FAU - Martinez Perez-Balsa, A AU - Martinez Perez-Balsa A FAU - Marti-Masso, J F AU - Marti-Masso JF FAU - Carrera, N AU - Carrera N FAU - Urtasun, M AU - Urtasun M LA - spa PT - Case Reports PT - English Abstract PT - Journal Article TT - Variabilidad clinica de los infartos talamicos paramedianos bilaterales. PL - SPAIN TA - Rev Neurol JT - Revista de neurologia JID - 7706841 SB - IM MH - Adult MH - Aged MH - Amnesia/etiology MH - Ataxia/etiology MH - Basal Ganglia Diseases/etiology MH - Cerebellum/pathology MH - *Cerebral Infarction/complications/pathology MH - Consciousness Disorders/etiology MH - Dysarthria/etiology MH - Female MH - Humans MH - Magnetic Resonance Imaging MH - Male MH - Middle Aged MH - Ocular Motility Disorders/etiology MH - Thalamus/*blood supply/pathology EDAT- 1998/02/12 MHDA- 1998/02/12 00:01 PST - ppublish SO - Rev Neurol. 1997 Sep;25(145):1353-62. PMID- 9379299 OWN - NLM STAT- MEDLINE DA - 19971112 DCOM- 19971112 LR - 20031114 PUBM- Print IS - 0022-3395 (Print) VI - 83 IP - 5 DP - 1997 Oct TI - Ascarophis marina n. comb. (Nematoda: cystidicolidae) from the fishes Parona signata (Carangidae) and Urophycis brasiliensis (Gadidae) in the southwestern Atlantic. PG - 917-21 AB - The taxonomic position of Cystidicola marina Szidat, 1961 is revised, based on the re-examination of type and new specimens collected from the type host, Urophycis brasiliensis (Gadidae), and a new host, Parona signata (Carangidae), in the southwestern Atlantic. The species is redescribed and transferred to Ascarophis as A. marina n. comb. It is distinguished from other species of Ascarophis by the following combination of characters: body length (male: 10.2-22.5 mm, female: 32.8-44.2 mm), number of egg filaments (2 on each pole), egg size (0.030-0.039 mm x 0.015-0.021 mm), and left spicule length (0.4-0.6 mm). AD - Centro de Estudios Parasitologicos y de Vectores (CEPAVE-CONICET), La Plata, Buenos Aires, Argentina. FAU - Ivanov, V A AU - Ivanov VA FAU - Navone, G T AU - Navone GT FAU - Martorelli, S R AU - Martorelli SR LA - eng PT - Journal Article PL - UNITED STATES TA - J Parasitol JT - The Journal of parasitology JID - 7803124 SB - IM MH - Animals MH - Female MH - Fish Diseases/*parasitology MH - Fishes MH - Male MH - Microscopy, Electron, Scanning MH - Nematoda/anatomy & histology/*classification/ultrastructure MH - Nematode Infections/parasitology/*veterinary EDAT- 1997/10/29 MHDA- 1997/10/29 00:01 PST - ppublish SO - J Parasitol. 1997 Oct;83(5):917-21. PMID- 9314714 OWN - NLM STAT- MEDLINE DA - 19971030 DCOM- 19971030 LR - 20061115 PUBM- Print IS - 0033-3182 (Print) VI - 38 IP - 5 DP - 1997 Sep-Oct TI - Use of medical services by veterans with mental disorders. PG - 451-8 AB - This study examined timeliness, access, and intensity of outpatient medical service use in a national sample of veterans with comorbid medical disorders discharged from Veterans Affairs (VA) psychiatric units (N = 44,533). The factors that predicted decreased use of medical services included diagnosis of schizophrenia, posttraumatic stress disorder, and substance abuse. The factors associated with increased use of medical services included proximity to a VA outpatient clinic, receipt of VA compensation payments, discharge from a facility with greater resources devoted to medical-surgical care, and prompt outpatient mental health follow-up. Better integration of medical and psychiatric services may help improve access to medical care for the severely mentally ill. AD - Northeast Program Evaluation Center, Department of Veterans Affairs, West Haven, Connecticut, USA. FAU - Druss, B G AU - Druss BG FAU - Rosenheck, R A AU - Rosenheck RA LA - eng GR - 15783/PHS PT - Journal Article PT - Research Support, U.S. Gov't, P.H.S. PL - UNITED STATES TA - Psychosomatics JT - Psychosomatics JID - 0376506 SB - IM MH - Adult MH - Aged MH - Ambulatory Care/utilization MH - Community Mental Health Services/*utilization MH - Comorbidity MH - Cross-Sectional Studies MH - Female MH - Health Services Accessibility/*statistics & numerical data MH - Humans MH - Incidence MH - Male MH - Mental Disorders/diagnosis/*epidemiology/psychology MH - Middle Aged MH - Patient Discharge/statistics & numerical data MH - United States MH - Veterans/*psychology/statistics & numerical data EDAT- 1997/10/07 MHDA- 1997/10/07 00:01 PST - ppublish SO - Psychosomatics. 1997 Sep-Oct;38(5):451-8. PMID- 9293538 OWN - NLM STAT- MEDLINE DA - 19971029 DCOM- 19971029 LR - 20041117 PUBM- Print IS - 1320-5463 (Print) VI - 47 IP - 8 DP - 1997 Aug TI - Ameloblastic fibrosarcoma arising de novo in the maxilla. PG - 564-8 AB - An ameloblastic fibrosarcoma (AFS) arising in the maxilla of a 14-year-old male is described. The tumor originated from the alveolar bone of the right maxilla with no apparent history of pre-existing lesion. Histologically, the lesion was composed of benign-appearing epithelial islands and strands scattered within an exceedingly cellular mass of mesenchymal tissue comprising a large number of stellate-and spindle-shaped fibroblast-like cells with marked pleomorphism. Occasional cementum-like calcification was also noted. Immunohistochemically, the neoplastic mesenchymal cells were positive only for vimentin, whereas the ameloblast-like epithelial component showed a distinctly positive reaction for wide-spectrum keratin and squamous cytokeratin. Clinicopathological features of the current case, as well as previously reported examples of AFS originating from the maxilla, are briefly discussed. AD - Department of Oral Pathology, Meikai University School of Dentistry, Saitama, Japan. FAU - Tajima, Y AU - Tajima Y FAU - Utsumi, N AU - Utsumi N FAU - Suzuki, S AU - Suzuki S FAU - Fujita, K AU - Fujita K FAU - Takahashi, H AU - Takahashi H LA - eng PT - Case Reports PT - Journal Article PL - AUSTRALIA TA - Pathol Int JT - Pathology international JID - 9431380 SB - IM MH - Adolescent MH - Biopsy MH - Fatal Outcome MH - Humans MH - Male MH - Maxilla/pathology MH - Maxillary Neoplasms/*pathology MH - Odontogenic Tumors/*pathology EDAT- 1997/08/01 00:00 MHDA- 1997/09/18 00:01 PST - ppublish SO - Pathol Int. 1997 Aug;47(8):564-8. PMID- 9346183 OWN - NLM STAT- MEDLINE DA - 19971114 DCOM- 19971114 LR - 20041117 PUBM- Print IS - 0893-3952 (Print) VI - 10 IP - 10 DP - 1997 Oct TI - Solitary fibrous tumor of soft tissue: a report of 15 cases, including 5 malignant examples with light microscopic, immunohistochemical, and ultrastructural data. PG - 1028-37 AB - We describe 15 soft tissue solitary fibrous tumors (SFTs) occurring in patients 24 to 78 years old (average, 50.6 yr). Ten tumors were benign and arose in the head and neck area (three tumors), thigh (two), vulva (two), upper arm (one), lower leg (one), and retroperitoneum (one). Five tumors were histologically malignant and arose in the thigh (two), abdominal wall (one), buttock (one), and retroperitoneum (one). All of the tumors were grossly well circumscribed. The benign tumors measured from 2 to 10 cm (average, 4.8 cm) and the malignant ones from 3 to 5.5 cm (average, 4.3 cm) in greatest diameter. Microscopically, the benign tumors showed areas of hypercellularity with variable amounts of collagenous and myxoid stroma; one had amianthoid fibers. The malignant tumors were composed of cytologically atypical cells enmeshed in a collagenous or myxoid extracellular matrix. Ultrastructural study of three benign and three malignant tumors showed fibroblastic differentiation; one benign tumor showed myofibroblastic differentiation. Immunohistochemically, all of the tumors examined were immunoreactive for vimentin, and seven of nine were positive for CD34, including all of the malignant ones. There was focal staining for muscle actin in two benign tumors and for Leu-7 in one benign tumor; there was no staining for cytokeratin, desmin, S-100 protein, epithelial membrane antigen, or smooth muscle actin in any of the examined tissues. Follow-up was available for eight patients for 6 to 21 months (average, 12 mo). No tumor recurred locally or metastasized. The SFTs reported herein support the experiences of others who recently described these tumors in the somatic soft tissues. In addition, our series highlights the occurrence of malignant SFTs in the soft tissues. SFTs should be separated from other spindle cell sarcomas, with which they can be confused. AD - The James Homer Wright Pathology Laboratories, Massachusetts General Hospital, Harvard Medical School, Boston 02114, USA. FAU - Nielsen, G P AU - Nielsen GP FAU - O'Connell, J X AU - O'Connell JX FAU - Dickersin, G R AU - Dickersin GR FAU - Rosenberg, A E AU - Rosenberg AE LA - eng PT - Case Reports PT - Journal Article PL - UNITED STATES TA - Mod Pathol JT - Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc JID - 8806605 RN - 0 (Tumor Markers, Biological) SB - IM MH - Abdominal Muscles/pathology MH - Adult MH - Aged MH - Buttocks/pathology MH - Cell Nucleus/ultrastructure MH - Endoplasmic Reticulum, Rough/ultrastructure MH - Extremities/pathology MH - Female MH - Fibroma/chemistry/*pathology/ultrastructure MH - Head and Neck Neoplasms/pathology MH - Humans MH - Immunohistochemistry MH - Male MH - Middle Aged MH - Retroperitoneal Neoplasms/pathology MH - Soft Tissue Neoplasms/chemistry/*pathology/ultrastructure MH - Tumor Markers, Biological/analysis MH - Vulvar Neoplasms/pathology EDAT- 1997/11/05 MHDA- 1997/11/05 00:01 PST - ppublish SO - Mod Pathol. 1997 Oct;10(10):1028-37. PMID- 9325071 OWN - NLM STAT- MEDLINE DA - 19971106 DCOM- 19971106 LR - 20061115 PUBM- Print IS - 0090-1229 (Print) VI - 85 IP - 1 DP - 1997 Oct TI - Plasma effects on phagocytic activity and hydrogen peroxide production by polymorphonuclear leukocytes in neonates. PG - 67-72 AB - To elucidate the defense mechanism in neonates against bacterial infections, phagocytic activity and hydrogen peroxide (H2O2) production by polymorphonuclear leukocytes (PMNs) in the whole blood and the effects of plasma on these functions were investigated on 44 healthy mature neonates (term 37 to 41 weeks) and 15 premature neonates (term 30 to 36 weeks) using two color flow cytometric analysis. The results were compared to those of a healthy adult control group (n = 10). PMN phagocytic activity was low in both mature and premature neonates. H2O2 production of PMN with phorbol myristate acetate (PMA) stimulation and following phagocytosis was augmented in both mature and premature neonates. When plasma and PMNs of adults and neonates were separated and combined differently, phagocytic activity and H2O2 production of PMNs appeared to be principally regulated by the plasma employed. This finding indicates that plasma has major effects on both phagocytosis and H2O2 production by PMNs of newborn neonates. AD - Department of Pediatrics, Kansai Medical University, Osaka, Japan. FAU - Fujiwara, T AU - Fujiwara T FAU - Kobayashi, T AU - Kobayashi T FAU - Takaya, J AU - Takaya J FAU - Taniuchi, S AU - Taniuchi S FAU - Kobayashi, Y AU - Kobayashi Y LA - eng PT - In Vitro PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - UNITED STATES TA - Clin Immunol Immunopathol JT - Clinical immunology and immunopathology JID - 0356637 RN - 16561-29-8 (Tetradecanoylphorbol Acetate) RN - 7722-84-1 (Hydrogen Peroxide) SB - IM MH - Adult MH - Age Factors MH - *Blood Bactericidal Activity MH - Fetal Blood/cytology/*immunology/*metabolism MH - Humans MH - Hydrogen Peroxide/*blood MH - Infant, Newborn MH - Infant, Premature MH - Neutrophils/drug effects/*immunology/*metabolism MH - *Phagocytosis MH - Plasma/*immunology MH - Tetradecanoylphorbol Acetate/pharmacology EDAT- 1997/11/05 MHDA- 1997/11/05 00:01 AID - S0090122997944032 [pii] PST - ppublish SO - Clin Immunol Immunopathol. 1997 Oct;85(1):67-72. PMID- 9379259 OWN - NLM STAT- MEDLINE DA - 19971107 DCOM- 19971107 LR - 20061115 PUBM- Print IS - 0736-0266 (Print) VI - 15 IP - 4 DP - 1997 Jul TI - Forearm muscle oxygenation decreases with low levels of voluntary contraction. PG - 507-11 AB - The purpose of our investigation was to determine if the near infrared spectroscopy technique was sensitive to changes in tissue oxygenation at low levels of isometric contraction in the extensor carpi radialis brevis muscle. Nine subjects were seated with the right arm abducted to 45 degrees, elbow flexed to 85 degrees, forearm pronated 45 degrees, and wrist and forearm supported on an armrest throughout the protocol. Altered tissue oxygenation was measured noninvasively with near infrared spectroscopy. The near infrared spectroscopy probe was placed over the extensor carpi radialis brevis of the subject's right forearm and secured with an elastic wrap. After 1 minute of baseline measurements taken with the muscle relaxed, four different loads were applied just proximal to the metacarpophalangeal joint such that the subjects isometrically contracted the extensor carpi radialis brevis at 5, 10, 15, and 50% of the maximum voluntary contraction for 1 minute each. A 3-minute recovery period followed each level of contraction. At the end of the protocol, with the probe still in place, a value for ischemic tissue oxygenation was obtained for each subject. This value was considered the physiological zero and hence 0% tissue oxygenation. Mean tissue oxygenation (+/-SE) decreased from resting baseline (100% tissue oxygenation) to 89 +/- 4, 81 +/- 8, 78 +/- 8, and 47 +/- 8% at 5, 10, 15, and 50% of the maximum voluntary contraction, respectively. Tissue oxygenation levels at 10, 15, and 50% of the maximum voluntary contraction were significantly lower (p < 0.05) than the baseline value. Our results indicate that tissue oxygenation significantly decreases during brief, low levels of static muscle contraction and that near infrared spectroscopy is a sensitive technique for detecting deoxygenation noninvasively at low levels of forearm muscle contraction. Our findings have important implications in occupational medicine because oxygen depletion induced by low levels of muscle contraction may be directly linked to muscle fatigue. AD - Ergonomics Laboratory, University of California, Berkeley, USA. FAU - Murthy, G AU - Murthy G FAU - Kahan, N J AU - Kahan NJ FAU - Hargens, A R AU - Hargens AR FAU - Rempel, D M AU - Rempel DM LA - eng PT - Journal Article PT - Research Support, U.S. Gov't, Non-P.H.S. PL - UNITED STATES TA - J Orthop Res JT - Journal of orthopaedic research : official publication of the Orthopaedic Research Society JID - 8404726 SB - IM SB - S MH - Adult MH - Exertion/physiology MH - Female MH - Forearm MH - Human Engineering MH - Humans MH - Male MH - Muscle Contraction/*physiology MH - Muscle Fatigue/physiology MH - Muscle, Skeletal/*physiology MH - Oxygen Consumption/*physiology MH - Spectroscopy, Near-Infrared MH - Volition/physiology OTO - NASA OT - NASA Center ARC OT - NASA Discipline Musculoskeletal IR - Hargens AR FIR - Hargens, A R IRAD- ARC EDAT- 1997/07/01 00:00 MHDA- 1997/10/23 00:01 AID - 10.1002/jor.1100150405 [doi] PST - ppublish SO - J Orthop Res. 1997 Jul;15(4):507-11. PMID- 9085387 OWN - NLM STAT- MEDLINE DA - 19971119 DCOM- 19971119 LR - 20041117 PUBM- Print IS - 0091-7435 (Print) VI - 26 IP - 2 DP - 1997 Mar-Apr TI - Educational and informational strategies to reduce pesticide risks. Council on Scientific Affairs. PG - 191-200 AB - BACKGROUND: In 1993, the American Medical Association (AMA) requested its Council on Scientific Affairs to investigate issues and concerns related to (1) improving public notification of pesticide applications and (2) improving educational programs for commercial and farm pesticide applicators and increasing enforcement of licensing examination requirements. This report was presented at the 1994 Interim Meeting of the AMA House of Delegates as Report 4 of the Council on Scientific Affairs. METHODS: Information for the report was derived from published literature and from personal communications with state and federal regulatory officials, physicians, and representatives of pest control, lawn care, and farm organizations. Some information about state certification and training programs was obtained from telephone conversations with pesticide applicator training program coordinators from California, Florida, Illinois, Iowa, Michigan, Missouri, Nebraska, New Jersey, New York, Texas, Washington, and Wisconsin. These states were selected because they contain large agricultural or urban populations that are likely to require the services of trained professional pesticide applicators. RESULTS: Current surveillance systems are inadequate to characterize potential exposure problems related either to pesticide usage or to pesticide related illnesses. The effectiveness of applicator certification and training programs and public notification programs could not be determined because of a lack of federal and state survey data for making useful assessments. CONCLUSIONS: Considering current data gaps, it is prudent for homeowners, farmers, and workers to limit pesticide exposures to themselves and others. LA - eng PT - Journal Article PT - Review PL - UNITED STATES TA - Prev Med JT - Preventive medicine JID - 0322116 RN - 0 (Pesticides) SB - IM MH - Certification/standards MH - Environmental Exposure/adverse effects/legislation & jurisprudence/*prevention & control MH - *Environmental Health/legislation & jurisprudence/standards MH - Government Agencies/legislation & jurisprudence/standards MH - Humans MH - Pest Control/standards MH - Pesticides/*adverse effects MH - Sentinel Surveillance MH - United States RF - 59 EDAT- 1997/03/01 MHDA- 1997/03/01 00:01 AID - S009174359690122X [pii] PST - ppublish SO - Prev Med. 1997 Mar-Apr;26(2):191-200. PMID- 9376959 OWN - NLM STAT- MEDLINE DA - 19971106 DCOM- 19971106 LR - 20051116 PUBM- Print IS - 1052-5157 (Print) VI - 7 IP - 4 DP - 1997 Oct TI - Angiographic evaluation and management of nonvariceal upper gastrointestinal bleeding. PG - 703-16 AB - Endoscopy is the primary diagnostic and therapeutic tool used in the evaluation and treatment of patients with upper gastrointestinal bleeding. When endoscopy is unsuccessful in identifying or controlling GI hemorrhage, however, arteriography is useful in both the evaluation and treatment of upper GI hemorrhage. AD - Dotter Interventional Institution, Oregon Health Sciences University, Portland, OR 97201, USA. FAU - Hamlin, J A AU - Hamlin JA FAU - Petersen, B AU - Petersen B FAU - Keller, F S AU - Keller FS FAU - Rosch, J AU - Rosch J LA - eng PT - Case Reports PT - Journal Article PT - Review PL - UNITED STATES TA - Gastrointest Endosc Clin N Am JT - Gastrointestinal endoscopy clinics of North America JID - 9202792 SB - IM MH - Adult MH - Angiography/*methods MH - Diagnosis, Differential MH - Esophageal and Gastric Varices/radiography/therapy MH - Gastrointestinal Hemorrhage/*radiography/therapy MH - Humans MH - Male MH - Middle Aged MH - Peptic Ulcer Hemorrhage/radiography/therapy MH - Postoperative Hemorrhage/radiography MH - Sensitivity and Specificity RF - 35 EDAT- 1998/02/12 MHDA- 1998/02/12 00:01 PST - ppublish SO - Gastrointest Endosc Clin N Am. 1997 Oct;7(4):703-16. PMID- 9327223 OWN - NLM STAT- MEDLINE DA - 19971120 DCOM- 19971120 LR - 20051116 PUBM- Print IS - 1040-8746 (Print) VI - 9 IP - 5 DP - 1997 Sep TI - HIV-associated lymphomas. PG - 450-4 AB - Intermediate and high-grade non-Hodgkin's lymphomas (NHL) with a B-cell phenotype are AIDS-defining illnesses. The incidence of systemic NHL increased greatly and primary central nervous system NHL increased even more in the HIV-infected population. Further increases in frequency are anticipated as HIV-infected individuals survive longer in an immunosuppressed state with improved antiretroviral treatment and treatment of opportunistic infections. Unusual types of NHL and manifestations of Hodgkin's disease are seen in HIV-infected individuals also. The pathologic and clinical features of the HIV-associated lymphomas and treatment approaches and results are the subjects of this review. Other articles in this issue discuss epidemiology and pathogenesis. AD - Memorial Sloan-Kettering Cancer Center, New York, NY 10021, USA. FAU - Straus, D J AU - Straus DJ LA - eng PT - Clinical Trial PT - Journal Article PT - Review PL - UNITED STATES TA - Curr Opin Oncol JT - Current opinion in oncology JID - 9007265 SB - IM SB - X MH - Central Nervous System Neoplasms/pathology MH - Humans MH - Lymphoma, AIDS-Related/*pathology/therapy MH - Lymphoma, Non-Hodgkin/pathology RF - 46 EDAT- 1997/11/05 MHDA- 1997/11/05 00:01 PST - ppublish SO - Curr Opin Oncol. 1997 Sep;9(5):450-4. PMID- 9339895 OWN - NLM STAT- MEDLINE DA - 19971114 DCOM- 19971114 LR - 20061115 PUBM- Print IS - 1016-8478 (Print) VI - 7 IP - 4 DP - 1997 Aug 31 TI - Hinnavin I, an antibacterial peptide from cabbage butterfly, Artogeia rapae. PG - 509-13 AB - We have previously isolated four antibacterial peptides from the immune haemolymph of the fifth instar larvae of cabbage butterfly, Artogeia rapae [Yoe, S. M., Bang, I. S., Kang, C. S., and Kim, H. J. (1996) Mol. Cells 6, 609-614]. They were induced by live, nonpathogenic gram negative bacteria. One of these novel antibacterial peptides was named hinnavin I. Hinnavin I is heat stable; its activity was retained after 60 min incubation at 100 degrees C, being effective against gram negative and/or gram positive bacteria. Hinnavin I and lysozyme II showed a powerful synergistic effect on the inhibition of bacterial growth. Amino acid composition was analyzed and the molecular weight was determined to be 4,139.94+/-10.91 Da by the ESI mass spectrometer. To elucidate the primary structure of hinnavin I, the amino acid sequence of this peptide was determined by N-terminal sequencing techniques. The amino-terminal half of the molecule was rich in charged amino acids and was hydrophilic, whereas the carboxyl-terminal half was hydrophobic. AD - Department of Biological Sciences, Dankook University, Cheonan, Korea. FAU - Bang, I S AU - Bang IS FAU - Son, S Y AU - Son SY FAU - Yoe, S M AU - Yoe SM LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - KOREA TA - Mol Cells JT - Molecules and cells JID - 9610936 RN - 0 (Amino Acids) RN - 0 (Anti-Bacterial Agents) RN - 0 (Growth Inhibitors) RN - 0 (Insect Proteins) RN - 0 (Peptides) RN - 0 (hinnavin I protein, Artogeia rapae) RN - EC 3.2.1.17 (Muramidase) SB - IM MH - Amino Acid Sequence MH - Amino Acids/analysis MH - Animals MH - Anti-Bacterial Agents/*chemistry/isolation & purification/pharmacology MH - Butterflies/*chemistry MH - Drug Synergism MH - Escherichia coli/drug effects/growth & development MH - Growth Inhibitors/pharmacology MH - Hemolymph/chemistry MH - Insect Proteins/*chemistry/isolation & purification/pharmacology MH - Molecular Sequence Data MH - Molecular Weight MH - Muramidase/pharmacology MH - Peptides/*chemistry/isolation & purification/pharmacology MH - Sequence Analysis EDAT- 1997/08/31 00:00 MHDA- 1997/10/27 00:01 PST - ppublish SO - Mol Cells. 1997 Aug 31;7(4):509-13. PMID- 9307842 OWN - NLM STAT- MEDLINE DA - 19971029 DCOM- 19971029 LR - 20061115 PUBM- Print IS - 0361-090X (Print) VI - 21 IP - 5 DP - 1997 TI - DNA adducts and the mechanism of carcinogenesis and cytotoxicity of methylating agents of environmental and clinical significance. PG - 391-405 AB - DNA adducts are covalent complexes formed between genotoxic carcinogens and DNA bases, and constitute a critical early intermediate on the pathway of chemical carcinogenesis. Their accumulation in different tissues reflects the amount of activated carcinogen reaching DNA, and can therefore serve as an index of the biologically relevant dose reaching the target tissues or cells. Methylating agents are of interest in view of their occurrence in the environment and their use as cytotoxic drugs in cancer chemotherapy. Current evidence indicates that O6-methylguanine plays a particularly important role in the mutagenic, carcinogenic, and cytotoxic activities of methylating agents. O6-Methylguanine is repaired efficiently by the enzyme O6-alkylguanine-DNA alkyltransferase (AGT). Lack of this enzyme results in excessive accumulation of O6-methylguanine and recent evidence suggests that significant quantitative effects on adduct accumulation may be linked to conditions of very low AGT levels. This would be important from the point of view of clinical practice, since modulation of AGT is under investigation as a means of enhancing the therapeutic efficacy of clinical agents acting via the production of O6-methylguanine and related adducts, such as, for example, procarbazine, dacarbazine, and some nitrosoureas. The measurement of O6-methylguanine in human DNA has been employed as a tool to investigate the role of environmental methylating agents in human carcinogenesis. While the nature and origin of the methylating agents responsible for these adducts is currently unknown, recent studies in patas monkeys have shown that N-nitrosodimethylamine, a methylating carcinogen to which human exposure is well documented, is capable of efficiently generating O6-methylguanine in most tissues, including fetal tissues. Furthermore, it has been found that this damage is substantially enhanced by the coadministration of ethyl alcohol which acts by inhibiting the liver first-pass metabolism of the carcinogen, an observation which supports the hypothesis that alcohol consumption may act as a risk factor in human carcinogenesis by augmenting the action of nitrosamines. AD - National Hellenic Research Foundation, Institute of Biological Research and Biotechnology, Athens, Greece. FAU - Kyrtopoulos, S A AU - Kyrtopoulos SA FAU - Anderson, L M AU - Anderson LM FAU - Chhabra, S K AU - Chhabra SK FAU - Souliotis, V L AU - Souliotis VL FAU - Pletsa, V AU - Pletsa V FAU - Valavanis, C AU - Valavanis C FAU - Georgiadis, P AU - Georgiadis P LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PT - Review PL - UNITED STATES TA - Cancer Detect Prev JT - Cancer detection and prevention JID - 7704778 RN - 0 (Antineoplastic Agents) RN - 0 (Carcinogens) RN - 0 (Carcinogens, Environmental) RN - 0 (DNA Adducts) RN - 0 (Nitroso Compounds) RN - 138-89-6 (4-nitrosodimethylaniline) RN - 20535-83-5 (O-(6)-methylguanine) RN - 62-75-9 (Dimethylnitrosamine) RN - 64-17-5 (Ethanol) RN - 671-16-9 (Procarbazine) RN - 73-40-5 (Guanine) SB - IM MH - Animals MH - Antineoplastic Agents/adverse effects/metabolism MH - Carcinogens/*metabolism/toxicity MH - Carcinogens, Environmental/metabolism/toxicity MH - DNA Adducts/*metabolism MH - DNA Damage MH - *DNA Methylation MH - Dimethylnitrosamine/metabolism/toxicity MH - Erythrocebus patas MH - Ethanol/pharmacology MH - Female MH - Guanine/analogs & derivatives/metabolism/toxicity MH - Humans MH - Leukocytes/drug effects MH - Liver/metabolism MH - Mice MH - Mice, Transgenic MH - Neoplasms/chemically induced/drug therapy MH - Nitroso Compounds/metabolism/toxicity MH - Procarbazine/metabolism/toxicity RF - 111 EDAT- 1997/01/01 00:00 MHDA- 1997/10/06 00:01 PST - ppublish SO - Cancer Detect Prev. 1997;21(5):391-405. PMID- 9385099 OWN - NLM STAT- MEDLINE DA - 19971125 DCOM- 19971125 LR - 20041117 PUBM- Print IS - 1380-7870 (Print) VI - 1 IP - 2 DP - 1995 TI - Product-limit survival functions with correlated survival times. PG - 171-86 AB - A simple variance estimator for product-limit survival functions is demonstrated for survival times with nested errors. Such data arise whenever survival times are observed within clusters of related observations. Greenwood's formula, which assumes independent observations, is not appropriate in this situation. A robust variance estimator is developed using Taylor series linearized values and the between-cluster variance estimator commonly used in multi-stage sample surveys. A simulation study shows that the between-cluster variance estimator is approximately unbiased and yields confidence intervals that maintain the nominal level for several patterns of correlated survival times. The simulation study also shows that Greenwood's formula underestimates the variance when the survival times are positively correlated within a cluster and yields confidence intervals that are too narrow. Extension to life table methods is also discussed. AD - Research Triangle Institute, NC 27709-2194, USA. willy@rti.org FAU - Williams, R L AU - Williams RL LA - eng PT - Journal Article PL - UNITED STATES TA - Lifetime Data Anal JT - Lifetime data analysis JID - 9516348 SB - IM MH - Angina Pectoris MH - Animals MH - Avoidance Learning MH - Exercise Test MH - Female MH - Humans MH - Life Tables MH - Linear Models MH - Male MH - Rodentia MH - *Survival Analysis EDAT- 1995/01/01 00:00 MHDA- 1998/02/12 00:01 PST - ppublish SO - Lifetime Data Anal. 1995;1(2):171-86. PMID- 9350074 OWN - NLM STAT- MEDLINE DA - 19971121 DCOM- 19971121 LR - 20061115 PUBM- Print IS - 0036-5513 (Print) VI - 57 IP - 6 DP - 1997 Oct TI - Minimal residual disease in B-lymphoproliferative disorders by PCR detection of immunoglobulin heavy chain gene recombination. PG - 541-7 AB - We amplified and sequenced the rearranged immunoglobulin heavy chain VDJ genomic unit in B-leukemias and used it as a clone-specific marker for the molecular monitoring of the patients during and after therapeutic treatment. The method described is patient-specific rather than disorder-specific, more sensitive and less time-consuming than other conventional techniques for the detection of minimal residual disease. We propose reproducible and quick procedures, from DNA extraction to Southern blotting, that can be easily performed in any clinical laboratory and also applied to other kinds of investigation. AD - Dipartimento di Scienze Biomediche e Oncologia Umana, Universita di Torino, Ospedale San Luigi Gonzaga, Turin, Italy. FAU - Petroni, D AU - Petroni D FAU - Saglio, G AU - Saglio G LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - NORWAY TA - Scand J Clin Lab Invest JT - Scandinavian journal of clinical and laboratory investigation JID - 0404375 RN - 0 (DNA, Neoplasm) RN - 0 (Immunoglobulin Heavy Chains) SB - IM MH - Base Sequence MH - Blotting, Southern MH - DNA, Neoplasm/analysis MH - Gene Rearrangement MH - Humans MH - Immunoglobulin Heavy Chains/*genetics MH - Leukemia, B-Cell, Chronic/*genetics/immunology MH - Leukemia, Hairy Cell/*genetics/immunology MH - Leukemia, Lymphocytic, Acute/*genetics/immunology MH - Molecular Sequence Data MH - Neoplasm, Residual/*diagnosis/genetics/immunology MH - *Polymerase Chain Reaction EDAT- 1997/11/14 MHDA- 1997/11/14 00:01 PST - ppublish SO - Scand J Clin Lab Invest. 1997 Oct;57(6):541-7. PMID- 9342465 OWN - NLM STAT- MEDLINE DA - 19971114 DCOM- 19971114 LR - 20041117 PUBM- Print IS - 0390-6663 (Print) VI - 24 IP - 2 DP - 1997 TI - Amniotic fluid index variations after amniocentesis, amnioinfusion and amnioreduction: preliminary data. PG - 70-3 AB - We studied the relationship between the ultrasonographically measurable variations in the amniotic fluid index (AFI) and actual changes in the amniotic fluid volume induced by three differing invasive procedures: genetic amniocentesis, amnioinfusion and amnioreduction. We examined 50 patients, all between the 15th and 34th weeks of pregnancy, subdivided into three groups. The first group consisted of 33 women who underwent genetic amniocentesis, the second was of 11 patients submitted to amnioinfusion for oligohydramnios (AFI < 5 cm), and the third was composed of 6 patients affected by hydramnios (AFI > 20 cm) and treated with amnioreduction. In all cases AFI was measured before and after the invasive procedures and their variations (delta AFI) were correlated to the actual quantities of liquid infused or extracted. All the procedures gave rise to statistically significant AFI changes. After genetic amniocentesis, the mean change was from 12.0 to 10.9 cm (p < 0.005), after amnioinfusion from 3.1 to 10.6 cm (p < 0.0001) and after amnioreduction from 33.1 to 22.0 cm. (p < 0.005). However, a significant linear correlation between delta AFI and the fluid volume variations actually induced was found for amnioinfusion (y = 0.236537 + 0.031465x; R2 = 44.4%; p < 0.05) and for amnioreduction (y = -0.0584294 + 0.012008x; R2 = 89.8%. p < 0.00001). Only for amnioreduction is it possible, as proved by a multiple regression analysis, to improve the predictability of delta AFI, taking into consideration together with the quantity of fluid aspirated, the value of the preprocedure AFI (R2 = 92%; p < 0.05). AD - Department of Obstetrics and Gynecology, University of Parma, Italy. FAU - Gramellini, D AU - Gramellini D FAU - Piantelli, G AU - Piantelli G FAU - Di Marino, O AU - Di Marino O FAU - Avanzini, A AU - Avanzini A FAU - Vadora, E AU - Vadora E LA - eng PT - Journal Article PL - ITALY TA - Clin Exp Obstet Gynecol JT - Clinical and experimental obstetrics & gynecology JID - 7802110 SB - IM MH - *Amniocentesis MH - Amniotic Fluid/*physiology/ultrasonography MH - Female MH - Humans MH - Oligohydramnios/*therapy MH - Polyhydramnios/*therapy MH - Pregnancy MH - Reference Values MH - Regression Analysis EDAT- 1997/01/01 00:00 MHDA- 1997/10/29 00:01 PST - ppublish SO - Clin Exp Obstet Gynecol. 1997;24(2):70-3. PMID- 9304708 OWN - NLM STAT- MEDLINE DA - 19971029 DCOM- 19971029 LR - 20061115 PUBM- Print IS - 0080-0015 (Print) VI - 144 DP - 1998 TI - Applications of gene transfer in hematologic malignancy. PG - 60-9 AB - Although gene transfer was originally conceived as a means to replace or correct defective genes in patients with inherited disorders, the process has shown broad potential for intervention in hematologic malignancy and for study of hematopoietic stem cell biology. Gene transfer strategies now under investigation for these applications include 1) repair of one or more genetic defects associated with the malignant process, 2) delivery of a prodrug-metabolizing enzyme that causes tumor cells to become sensitive to the corresponding anticancer drug, 3) modification of immune responses to the cancer, and 4) introduction of drug resistance genes to increase the therapeutic index of cytotoxic agents. Finally, by marking normal or malignant cells with readily detectable genes, one can monitor the efficacy of therapy or study the dynamics of stem cell behavior in vivo. At present these applications are limited by the quality of vectors, but as transduction efficiencies and gene regulatory mechanisms improve, gene transfer can be expected to evolve into a major therapeutic modality in its own right. AD - Cell and Gene Therapy Program, St. Jude Children's Research Hospital, Memphis, TN 38105, USA. FAU - Brenner, M K AU - Brenner MK LA - eng GR - CA 20180/CA/NCI GR - CA 21765/CA/NCI PT - Journal Article PT - Research Support, Non-U.S. Gov't PT - Research Support, U.S. Gov't, P.H.S. PT - Review PL - GERMANY TA - Recent Results Cancer Res JT - Recent results in cancer research. Fortschritte der Krebsforschung. Progres dans les recherches sur le cancer JID - 0044671 SB - IM MH - Drug Resistance, Neoplasm MH - *Gene Therapy MH - *Gene Transfer Techniques MH - Hematologic Neoplasms/genetics/immunology/*therapy MH - Hematopoietic Stem Cells MH - Humans MH - T-Lymphocytes, Cytotoxic/immunology RF - 37 EDAT- 1997/09/26 MHDA- 1997/09/26 00:01 PST - ppublish SO - Recent Results Cancer Res. 1998;144:60-9. PMID- 9365353 OWN - NLM STAT- MEDLINE DA - 19971125 DCOM- 19971125 LR - 20061115 PUBM- Print IS - 0003-9993 (Print) VI - 78 IP - 11 DP - 1997 Nov TI - Concentric and eccentric isokinetic assessment of flexor-extensor torque ratios at the hip, knee, and ankle in a sample population of healthy subjects. PG - 1224-30 AB - OBJECTIVE: To establish the relationship between the flexor/extensor torque ratios in the hip, knee, and ankle. DESIGN: Case series. SETTING: Laboratory of a university rehabilitation department. PARTICIPANTS: From a group of 158 healthy volunteers, 138 subjects completed all the tests in concentric mode, and 65 in eccentric mode. MAIN OUTCOME MEASURE: The flexor/extensor torque ratios of the hip, knee, and ankle were analyzed by means of isokinetic concentric and eccentric tests. Analysis of variance was carried out to compare the mean values of the ratios obtained between the male and female populations and between the right and left sides, and correlation analysis between the values of the joints. RESULTS: The flexor/extensor torque ratios differed significantly according to sex and angular velocities, but not according to side except for the ankle. No significant relationship was found between the flexor/extensor torque ratios in the hip, knee, and ankle joints. CONCLUSIONS: The flexor-extensor torque ratio of the knee and hip can be used as a reference point during rehabilitation of the contralateral side. Our results demonstrating the absence of correlation between the flexor/extensor torque ratio in each joint of the same limb, however, call for further longitudinal studies to be made under specific circumstances, such as training or immobilization of one joint, to follow the course of agonist/antagonist ratios and the synergistic activity between the joints. AD - Department of Physical Medicine and Rehabilitation, Lisfranc School of Medicine, Saint-Etienne University, France. FAU - Calmels, P M AU - Calmels PM FAU - Nellen, M AU - Nellen M FAU - van der Borne, I AU - van der Borne I FAU - Jourdin, P AU - Jourdin P FAU - Minaire, P AU - Minaire P LA - eng PT - Clinical Trial PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - UNITED STATES TA - Arch Phys Med Rehabil JT - Archives of physical medicine and rehabilitation JID - 2985158R SB - AIM SB - IM SB - S MH - Adult MH - Aged MH - Analysis of Variance MH - Ankle Joint/*physiology MH - Biomechanics MH - Exercise Test MH - Female MH - Hip Joint/*physiology MH - Humans MH - Knee Joint/*physiology MH - Male MH - Middle Aged MH - Muscle Contraction/*physiology MH - Physical Medicine/methods MH - Posture/physiology MH - Range of Motion, Articular/physiology MH - Reference Values MH - Reproducibility of Results MH - Tensile Strength/physiology MH - Torque EDAT- 1997/11/20 MHDA- 1997/11/20 00:01 AID - S0003-9993(97)90336-1 [pii] PST - ppublish SO - Arch Phys Med Rehabil. 1997 Nov;78(11):1224-30. PMID- 9327207 OWN - NLM STAT- MEDLINE DA - 19971103 DCOM- 19971103 LR - 20051116 PUBM- Print IS - 1062-4821 (Print) VI - 6 IP - 5 DP - 1997 Sep TI - The rise and fall of atrial natriuretic peptide for acute renal failure. PG - 474-6 AB - Atrial natriuretic peptide can increase glomerular filtration rate and filtration fraction and can promote natriuresis, effects that would logically seem to improve renal function after acute tubular necrosis from ischemic or toxic injury. Early human trials suggested a beneficial effect of atrial natriuretic peptide on creatinine clearance, and a reduction in the need for dialysis in treated patients. However, randomized, placebo-controlled trials have failed to show a clinically relevant benefit on survival, dialysis-free survival, or renal function in patients treated with this agent. AD - Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA. FAU - Brenner, R M AU - Brenner RM FAU - Chertow, G M AU - Chertow GM LA - eng PT - Journal Article PT - Review PL - UNITED STATES TA - Curr Opin Nephrol Hypertens JT - Current opinion in nephrology and hypertension JID - 9303753 RN - 85637-73-6 (Atrial Natriuretic Factor) SB - IM MH - Atrial Natriuretic Factor/*therapeutic use MH - Clinical Trials MH - Humans MH - Kidney Failure, Acute/*drug therapy MH - Randomized Controlled Trials RF - 24 EDAT- 1997/11/05 MHDA- 1997/11/05 00:01 PST - ppublish SO - Curr Opin Nephrol Hypertens. 1997 Sep;6(5):474-6. PMID- 9324068 OWN - NLM STAT- MEDLINE DA - 19971024 DCOM- 19971024 LR - 20061115 PUBM- Print IS - 0024-3205 (Print) VI - 61 IP - 13 DP - 1997 TI - Transplacental transfer of naltrexone in rats. PG - 1261-7 AB - Extracts of fetal (20 days gestation) brain, heart, and liver were evaluated for naltrexone in rats 1 hour following maternal injection of 50 mg/kg opioid antagonist; adult plasma from the pregnant rats was analyzed. Samples were prepared by ultrafiltration, lyophilized, reconstituted in mobile phase, and separated by reversed phase high-performance liquid chromatography with ultraviolet detection. This qualitative analysis revealed the presence of naltrexone in all fetal tissues, as well as in adult plasma. These results indicate naltrexone, maternally administered, passes through the placenta and enters the fetus. The data would suggest that reports concerning somatic and neurobiological acceleration in offspring exposed to naltrexone during gestation may be the result of a direct opioid antagonist action in the fetus. AD - Department of Neuroscience and Anatomy, The Pennsylvania State University College of Medicine, Hershey 17033, USA. FAU - Zagon, I S AU - Zagon IS FAU - Hurst, W J AU - Hurst WJ FAU - McLaughlin, P J AU - McLaughlin PJ LA - eng GR - HL-53557/HL/NHLBI GR - NS-20500/NS/NINDS PT - Journal Article PT - Research Support, U.S. Gov't, P.H.S. PL - ENGLAND TA - Life Sci JT - Life sciences JID - 0375521 RN - 0 (Narcotic Antagonists) RN - 16590-41-3 (Naltrexone) SB - IM MH - Animals MH - Brain/embryology/metabolism MH - Chromatography, High Pressure Liquid MH - Female MH - Heart/embryology MH - Kinetics MH - Liver/embryology/metabolism MH - Male MH - *Maternal-Fetal Exchange MH - Myocardium/metabolism MH - Naltrexone/*pharmacokinetics MH - Narcotic Antagonists/*pharmacokinetics MH - Placenta/*metabolism MH - Pregnancy MH - Rats MH - Rats, Sprague-Dawley EDAT- 1997/01/01 00:00 MHDA- 1997/11/04 00:01 AID - S0024320597006711 [pii] PST - ppublish SO - Life Sci. 1997;61(13):1261-7. PMID- 9322764 OWN - NLM STAT- MEDLINE DA - 19971030 DCOM- 19971030 LR - 20061115 PUBM- Print IS - 0378-1119 (Print) VI - 196 IP - 1-2 DP - 1997 Sep 1 TI - Prevalence and chromosomal map location of Staphylococcus aureus adhesin genes. PG - 249-59 AB - Using genomic DNA from 25 unrelated strains and probes specific for each gene, we assessed the prevalence of the Staphylococcus aureus (Sa) adhesion genes cna, fnbA, fnbB, fib, clfA, fbpA, ebpS and map. All 25 strains encoded fib, clfA, ebpS, map and at least one of the fnb genes. fbpA and coa appeared to be allelic variants of the same gene with the fbpA variant being present in only four of 25 isolates. cna was present in 10 of 25 strains. Using Southern blot analysis of SmaI-digested genomic DNA resolved by pulsed-field gel electrophoresis, the adhesion genes were mapped to SmaI fragments A (ebpS), B (fib and clfA), C (fnbA/fnbB), E (fbpA), F (map) and G (cna). Despite variations in SmaI restriction profiles, co-localization of adhesin genes with genes known to map to specific SmaI fragments in the Sa 8325-4 chromosome strains suggests that the chromosomal location of each adhesin gene is conserved. AD - Department of Microbiology and Immunology, University of Arkansas for Medical Sciences, Little Rock 72205, USA. msmeltzer@biomed.uams.edu FAU - Smeltzer, M S AU - Smeltzer MS FAU - Gillaspy, A F AU - Gillaspy AF FAU - Pratt, F L Jr AU - Pratt FL Jr FAU - Thames, M D AU - Thames MD FAU - Iandolo, J J AU - Iandolo JJ LA - eng GR - AI 37729/AI/NIAID PT - Journal Article PT - Research Support, U.S. Gov't, P.H.S. PL - NETHERLANDS TA - Gene JT - Gene JID - 7706761 RN - 0 (Adhesins, Bacterial) RN - 0 (Bacterial Proteins) RN - 0 (Carrier Proteins) RN - 0 (Fib protein, Staphylococcus aureus) RN - 0 (Receptors, Cell Surface) RN - 0 (adhesin, Staphylococcus aureus) RN - 0 (elastin-binding proteins) RN - EC 3.1.21.- (endodeoxyribonuclease XmaI) RN - EC 3.1.21.4 (Deoxyribonucleases, Type II Site-Specific) SB - IM MH - Adhesins, Bacterial/*genetics MH - Bacterial Proteins/genetics MH - Carrier Proteins/genetics MH - Chromosome Mapping MH - *Chromosomes, Bacterial MH - Deoxyribonucleases, Type II Site-Specific/genetics/metabolism MH - Receptors, Cell Surface/genetics MH - Staphylococcus aureus/*genetics EDAT- 1997/10/10 16:17 MHDA- 2001/03/28 10:01 PST - ppublish SO - Gene. 1997 Sep 1;196(1-2):249-59. PMID- 9237552 OWN - NLM STAT- MEDLINE DA - 19971028 DCOM- 19971028 LR - 20061115 PUBM- Print IS - 0006-8993 (Print) VI - 760 IP - 1-2 DP - 1997 Jun 20 TI - Iron deposits in multiple sclerosis and Alzheimer's disease brains. PG - 298-303 AB - Iron may contribute to the pathogenesis of neurological diseases by promoting oxidative damage. The localization of iron in multiple sclerosis (MS) and Alzheimer's disease (AD) brains was investigated to further the understanding of its pathogenic role in these disease states. Earlier studies, utilizing a standard Perls' stain, yielded conflicting reports regarding the distribution of iron deposits in MS brains, and a previous study on AD brains utilized a diaminobenzidine (DAB) enhanced version of this stain. In the present study, a modified version of the DAB-enhanced stain was used; it utilizes sodium borohydride, proteinase K, Triton X-100 and xylenes to increase the accessibility of tissue iron to histochemical reagents. This modified method can reveal iron deposits that are missed by the Perls' or DAB-enhanced Perls' stains. In addition to its normal deposition in oligodendrocytes and myelin, iron was detected in reactive microglia, ameboid microglia and macrophages in MS brains. In AD brains, three types of plaques were stained: dense core, clear core and amorphous plaques. Punctate staining was also observed in neurons in the corticies of AD brains. The structure accounting for punctate labeling may be damaged mitochondria, lipofuscin or amyloid deposits. Dense core plaques, clear plaques and punctate labeling were not detected in the previous AD study which utilized only the DAB-enhanced Perls' stain. The labeling of these additional structures illustrates the benefit of the modified method. In summary, the localization of iron deposition in MS and AD brains indicates potential sites where iron could promote oxidative damage in these disease states. AD - Department of Physiology and the Smith Mental Retardation Research Center, University of Kansas Medical Center, Kansas City 66160, USA. slevine@kumc.edu FAU - LeVine, S M AU - LeVine SM LA - eng GR - HD 02528/HD/NICHD GR - NS 33596/NS/NINDS PT - Journal Article PT - Research Support, Non-U.S. Gov't PT - Research Support, U.S. Gov't, Non-P.H.S. PT - Research Support, U.S. Gov't, P.H.S. PL - NETHERLANDS TA - Brain Res JT - Brain research JID - 0045503 RN - 7439-89-6 (Iron) SB - IM MH - Alzheimer Disease/*pathology MH - Brain/*pathology MH - Humans MH - Iron/*metabolism MH - Multiple Sclerosis/*pathology EDAT- 1997/06/20 MHDA- 1997/06/20 00:01 AID - S0006-8993(97)00470-8 [pii] PST - ppublish SO - Brain Res. 1997 Jun 20;760(1-2):298-303. PMID- 9344613 OWN - NLM STAT- MEDLINE DA - 19971125 DCOM- 19971125 LR - 20061115 PUBM- Print IS - 0014-4827 (Print) VI - 236 IP - 1 DP - 1997 Oct 10 TI - Prostaglandin H synthase expression is variable in human colorectal adenocarcinoma cell lines. PG - 321-9 AB - The expression of prostaglandin H synthases can be induced by many stimuli and is likely to be important in control of the cell cycle. The analysis of prostaglandin H synthase-1 and -2 expression in colon adenocarcinoma cell lines is a useful model system for studying the function of the prostaglandin H synthases, especially with regard to proliferation and adhesion. Prostaglandin H synthase-1 protein is not found in any of eight human colon adenocarcinoma cell lines. Expression of prostaglandin H synthase-2 is variable for the eight cell lines: three constitutively expressed active protein, four did not express this gene at all, and one had mRNA but no active protein. Thus, five colorectal adenocarcinoma cell lines exhibit "null" expression of prostaglandin synthase-2. The three cell lines with constitutive expression of prostaglandin H synthase-2 produce PGE2. Prostaglandin E2 production could be inhibited by aspirin and NS398 without inhibiting proliferation, while direct addition of prostaglandin E2 inhibits proliferation. Adhesion to collagen IV and fibronectin was stronger in those cell lines that expressed prostaglandin H synthase-2. The constitutive expression of prostaglandin H synthase-2 is associated with increased adhesion to extracellular matrix components and a potential inhibition of proliferation through the production of prostaglandin E2. The absence of PGH synthase-2 expression in some cell lines may result from the original tumor's need to inactivate these associated functions. Our evidence suggests that PGH synthase-2 is a possible candidate for a tumor suppressor gene at 1q23-qter. AD - Division of Hematology/Oncology, James H. Quillen College of Medicine, East Tennessee State University, Johnson City 37614-0622, USA. FAU - Parker, J AU - Parker J FAU - Kaplon, M K AU - Kaplon MK FAU - Alvarez, C J AU - Alvarez CJ FAU - Krishnaswamy, G AU - Krishnaswamy G LA - eng PT - Comparative Study PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - UNITED STATES TA - Exp Cell Res JT - Experimental cell research JID - 0373226 RN - 0 (Cyclooxygenase Inhibitors) RN - 0 (DNA, Neoplasm) RN - 0 (Extracellular Matrix Proteins) RN - 0 (Nitrobenzenes) RN - 0 (Prostaglandins) RN - 0 (RNA, Messenger) RN - 0 (Sulfonamides) RN - 123653-11-2 (N-(2-cyclohexyloxy-4-nitrophenyl)methanesulfonamide) RN - 50-78-2 (Aspirin) RN - EC 1.14.99.1 (Prostaglandin-Endoperoxide Synthases) SB - IM MH - Adult MH - Aged MH - Aspirin/pharmacology MH - Blotting, Southern MH - Caco-2 Cells/cytology/*enzymology MH - Cell Adhesion/physiology MH - Cell Division/physiology MH - Cyclooxygenase Inhibitors/pharmacology MH - DNA, Neoplasm/analysis MH - Extracellular Matrix Proteins/metabolism MH - Female MH - Gene Expression Regulation, Enzymologic/drug effects/physiology MH - Gene Expression Regulation, Neoplastic/drug effects/physiology MH - HT29 Cells/cytology/*enzymology MH - Humans MH - Male MH - Middle Aged MH - Nitrobenzenes/pharmacology MH - Prostaglandin-Endoperoxide Synthases/*genetics/metabolism MH - Prostaglandins/biosynthesis MH - RNA, Messenger/metabolism MH - Sulfonamides/pharmacology EDAT- 1997/11/05 MHDA- 1997/11/05 00:01 AID - S0014-4827(97)93741-1 [pii] AID - 10.1006/excr.1997.3741 [doi] PST - ppublish SO - Exp Cell Res. 1997 Oct 10;236(1):321-9. PMID- 9345238 OWN - NLM STAT- MEDLINE DA - 19971121 DCOM- 19971121 LR - 20061115 PUBM- Print IS - 1078-5884 (Print) VI - 14 IP - 3 DP - 1997 Sep TI - A paper for debate: vein versus PTFE for critical limb ischaemia--an unfair comparison? PG - 191-4 AB - INTRODUCTION: There is a widely held view that vein grafts for infrainguinal arterial reconstruction perform much better than prosthetic conduits, the best of which seems to be PTFE. Many randomised studies have been conducted which confirm this opinion, but is the difference as large as it is thought to be? One interesting feature of published trials is that the results for obligatory PTFE (when no vein is available) were much worse than the results for randomised PTFE grafts. The only way to explain this is that these groups of patients were not similar, and there are probably other factors which contribute to the difference in results when vein and PTFE grafts are compared. MATERIALS AND METHODS: A consecutive series of 109 femoro-infrapopliteal grafts undertaken for critical limb ischaemia was analysed to see the difference between vein and PTFE with vein cuff grafts. RESULTS: Vein grafts were superior to PTFE grafts when the whole cohort was included (p = 0.0038); however, there was no significant difference when the patients were stratified for inflow and runoff status. CONCLUSIONS: The difference between vein and PTFE has probably been exaggerated in the past, due to differences in risk factors and in the extent of arterial disease between the two groups of patients. The advantage of vein becomes more significant with time. AD - Department of Surgery, Guy's Hospital, London, U.K. FAU - Panayiotopoulos, Y P AU - Panayiotopoulos YP FAU - Taylor, P R AU - Taylor PR LA - eng PT - Comparative Study PT - Journal Article PL - ENGLAND TA - Eur J Vasc Endovasc Surg JT - European journal of vascular and endovascular surgery : the official journal of the European Society for Vascular Surgery JID - 9512728 RN - 9002-84-0 (Polytetrafluoroethylene) SB - IM MH - Blood Vessel Prosthesis MH - *Blood Vessel Prosthesis Implantation MH - Cohort Studies MH - Graft Occlusion, Vascular/epidemiology MH - Humans MH - Ischemia/*surgery MH - Leg/*blood supply MH - *Polytetrafluoroethylene MH - Risk Factors MH - Treatment Outcome MH - Vascular Patency MH - Veins/*transplantation EDAT- 1997/11/05 MHDA- 1997/11/05 00:01 PST - ppublish SO - Eur J Vasc Endovasc Surg. 1997 Sep;14(3):191-4. PMID- 9336588 OWN - NLM STAT- MEDLINE DA - 19971113 DCOM- 19971113 LR - 20041117 PUBM- Print IS - 0002-8223 (Print) VI - 97 IP - 10 Suppl 2 DP - 1997 Oct TI - The ketogenic diet revisited. PG - S192-4 AB - The ketogenic diet is a high-fat diet that maintains the body's starvation mechanism, with exogenous fat provided for metabolism in lieu of stored fat. Mild dehydration is important to prevent dilution of the level of ketones in circulation at any given time. It is not known why or how ketosis affects seizure activity, so the principles behind the therapy have been developed from years of clinical experience and theoretical assumptions. Dietitians are essential providers of ketosis therapy, but the dietitian must work with a physician who understands the theories behind the therapy and is an active member of the ketosis therapy team. AD - Hermann Children's Hospital, University of Texas Medical School, Houston, USA. FAU - Berryman, M S AU - Berryman MS LA - eng PT - Journal Article PL - UNITED STATES TA - J Am Diet Assoc JT - Journal of the American Dietetic Association JID - 7503061 SB - AIM SB - IM MH - Diet/adverse effects/*methods MH - Humans MH - Ketosis/*etiology MH - Seizures/*diet therapy EDAT- 1997/10/23 MHDA- 1997/10/23 00:01 PST - ppublish SO - J Am Diet Assoc. 1997 Oct;97(10 Suppl 2):S192-4. PMID- 9336566 OWN - NLM STAT- MEDLINE DA - 19971113 DCOM- 19971113 LR - 20041117 PUBM- Print IS - 0002-8223 (Print) VI - 97 IP - 10 Suppl 2 DP - 1997 Oct TI - Research competencies in the dietetics curricula. PG - S102-6 AB - Investment in dietetics research has the potential to improve general health; increase work performance and learning capacity; extend disease-free life; reduce birth defects, infections, and chronic diseases; and decrease health care costs. Opportunities exist for research in the areas of solid waste management, global environment, health care reform, and foodservice systems management. Research efforts can focus on cost-effectiveness and medical efficacy of dietary services to improve third-party reimbursement. Educators have a stake in creating the future by conducting research, teaching research to dietetic students, and investigating the effectiveness of education. AD - Veterans Affairs New Jersey Health Care Systems, East Orange 07108-1095, USA. FAU - Hynak-Hankinson, M T AU - Hynak-Hankinson MT FAU - Martin, S AU - Martin S FAU - Wirth, J AU - Wirth J LA - eng PT - Journal Article PL - UNITED STATES TA - J Am Diet Assoc JT - Journal of the American Dietetic Association JID - 7503061 SB - AIM SB - IM MH - Clinical Competence MH - *Curriculum MH - Data Collection/methods MH - Dietetics/*education MH - Humans MH - *Research MH - United States EDAT- 1997/10/23 MHDA- 1997/10/23 00:01 PST - ppublish SO - J Am Diet Assoc. 1997 Oct;97(10 Suppl 2):S102-6. PMID- 9323515 OWN - NLM STAT- MEDLINE DA - 19971120 DCOM- 19971120 LR - 20051117 PUBM- Print IS - 0392-0461 (Print) VI - 18 IP - 4 DP - 1997 Aug TI - Recurrent external ophthalmoparesis during hormonal therapy after thyroid ablation. Case report. PG - 215-6 AB - We here report the case of a patient who had undergone total thyroid ablation for Graves' disease. After the beginning of oral therapy with 1-thyroxine, she developed a left external ophthalmoparesis that remitted with the discontinuation of the drug and recurred whenever the replacement therapy (1-thyroxine or tri-iodothyronine) was reintroduced. AD - Divisione di Neurologia, Ospedale Civile di Belluno, Italy. FAU - Ferracci, F AU - Ferracci F FAU - Conte, F AU - Conte F FAU - Marini, B AU - Marini B FAU - Fassetta, G AU - Fassetta G LA - eng PT - Case Reports PT - Journal Article PL - ITALY TA - Ital J Neurol Sci JT - Italian journal of neurological sciences JID - 8006502 RN - 0 (Thyroid Hormones) SB - IM MH - Aged MH - Combined Modality Therapy MH - Female MH - Graves Disease/drug therapy/surgery/*therapy MH - Humans MH - Recurrence MH - Thyroid Gland/*surgery MH - Thyroid Hormones/*therapeutic use EDAT- 1997/08/01 00:00 MHDA- 1997/11/05 00:01 PST - ppublish SO - Ital J Neurol Sci. 1997 Aug;18(4):215-6. PMID- 9380370 OWN - NLM STAT- MEDLINE DA - 19971110 DCOM- 19971110 LR - 20061115 PUBM- Print IS - 1040-5488 (Print) VI - 74 IP - 9 DP - 1997 Sep TI - Evaluation of Kojima-Matsubara color vision test plates: validity in young children. PG - 726-31 AB - PURPOSE: We examined a pseudoisochromatic color plate test by Kojima and Matsubara for young children which uses drawings of familiar objects rather than letters or numbers. First, we evaluated the test's efficacy as a color deficiency screener and its validity in classifying the types of color deficiencies by comparing its results with those from the Moreland anomaloscope. Second, we eliminated the chromatic factor and evaluated the functional ability of young children to perform the task by determining how many correct responses were obtained using modified black/white replicas of the test plates. METHODS: Part 1: Twenty color-normal and 13 color-deficient adults were diagnosed and classified with the Ishihara test, Panel D-15 test, and anomaloscope. Subjects were then tested with the Kojima-Matsubara test and result were compared with those from the anomaloscope. Part 2: Fifty children aged 3 to 7 years were tested with modified black/white test plate replicas. The number of correct responses for each plate was determined for five different age groups. RESULTS: Part 1: Among the 20 color-normal subjects, 18 read all 10 plates correctly and 2 subjects missed 1 of the 10. Only 1 of the 13 color-deficient subjects exhibited the expected responses for plates 2 to 6 (used for color deficiency screening). The color-deficient subjects' responses for plates 7 to 10, which are used to classify red-green defects, were varied and only the protanomalous subjects (n = 2) followed the expected response pattern. Part 2: Of the 10 black/white modified plates, only 2 were correctly identified by all 50 children. The other plates had a recognition rate that ranged from 32 to 98%. CONCLUSIONS: Because the response patterns given by most of the color-deficient adult subjects were different from those in the test manual, ambiguous results would occur if the Kojima-Matsubara test were used for color vision screening or the diagnosis of color deficiency. In addition, the difficulty that many of the young children exhibited in identifying the objects in the black/white replica plates suggests that there would be a large number of false positive errors (classifying a color normal as color deficient) when using this test in young children. AD - Illinois College of Optometry, Chicago, USA. FAU - Lee, D Y AU - Lee DY FAU - Cotter, S A AU - Cotter SA FAU - French, A L AU - French AL LA - eng PT - Comparative Study PT - Journal Article PL - UNITED STATES TA - Optom Vis Sci JT - Optometry and vision science : official publication of the American Academy of Optometry JID - 8904931 SB - IM MH - Adult MH - Aging/physiology MH - Child MH - Child, Preschool MH - Color Perception/*physiology MH - Color Perception Tests/*methods MH - Color Vision Defects/diagnosis MH - Evaluation Studies MH - Humans MH - Reproducibility of Results MH - Vision Screening/*methods EDAT- 1997/11/05 MHDA- 1997/11/05 00:01 PST - ppublish SO - Optom Vis Sci. 1997 Sep;74(9):726-31. PMID- 9333208 OWN - NLM STAT- MEDLINE DA - 19971120 DCOM- 19971120 LR - 20061115 PUBM- Print IS - 0031-9384 (Print) VI - 62 IP - 5 DP - 1997 Nov TI - Behavioral and physiological sex differences observed in an animal model of fulminant hepatic encephalopathy in the rat. PG - 1113-24 AB - Hepatic encephalopathy is characterized by a number of neuropsychiatric and motor disturbances observed in patients with liver dysfunction. The purpose of this study is to fully characterize behavioral and physiological sex differences in an animal model of fulminant hepatic encephalopathy (FHE). Male and female rats were administered thioacetamide (600 mg/kg) via i.p. (intraperitoneal) injection at Hours 0 and 24 and allowed to progress into the four stages of FHE. Male rats reached all four stages of FHE significantly earlier than female rats (p < 0.05). The performance of the male rats deteriorated more quickly (p < 0.05) than that of the females in all of the sensory and motor behavioral tests. Sex differences were observed in the liver enzymes of the FHE rats. The levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), and alkaline phosphatase were significantly greater (p < 0.05) in male rats in all four stages of FHE. Significant increases were also observed in the levels of direct and total bilirubin (p < 0.05). Neuronal damage was observed in the CA1 and CA2 regions of the hippocampus. In the CA1 region, male rats displayed greater pathological changes in Stages III and IV (p < 0.05) than female rats. The damage in the CA2 region was only observed in Stage IV male rats. Our data indicate that observable behavioral and physiological sex differences occur in thioacetamide-induced FHE in the rat. AD - Department of Oral Biology, School of Dentistry, Creighton University, Omaha, NE 68178, USA. Nsnorton@creighton.edu FAU - Norton, N S AU - Norton NS FAU - McConnell, J R AU - McConnell JR FAU - Rodriguez-Sierra, J F AU - Rodriguez-Sierra JF LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - UNITED STATES TA - Physiol Behav JT - Physiology & behavior JID - 0151504 RN - 62-55-5 (Thioacetamide) SB - IM MH - Animals MH - Behavior, Animal/drug effects/*physiology MH - Brain Mapping MH - Female MH - Hepatic Encephalopathy/chemically induced/pathology/*physiopathology MH - Hippocampus/drug effects/pathology/physiopathology MH - Injections, Intraperitoneal MH - *Liver Function Tests MH - Male MH - Neurons/drug effects/pathology/physiology MH - Rats MH - Rats, Sprague-Dawley MH - Sex Factors MH - Thioacetamide EDAT- 1997/10/23 MHDA- 1997/10/23 00:01 AID - S0031-9384(97)00267-9 [pii] PST - ppublish SO - Physiol Behav. 1997 Nov;62(5):1113-24. PMID- 9297630 OWN - NLM STAT- MEDLINE DA - 19971124 DCOM- 19971124 LR - 20061115 PUBM- Print IS - 0895-8696 (Print) VI - 8 IP - 3 DP - 1997 Jun TI - Corticotropin-releasing factor and glucocorticoid receptor (GR) gene expression in the paraventricular nucleus of immune-challenged transgenic mice expressing type II GR antisense ribonucleic acid. PG - 165-79 AB - The purpose of this study was to investigate the effect of the immune activator lipopolysaccharide (LPS) on the expression of corticotropin-releasing factor (CRF) and glucocorticoid receptor (GR) mRNA in the paraventricular nucleus (PVN) of transgenic mice with impaired GR function caused by endogenous expression of GR antisense RNA. At 3 and 8 wk of age, control and transgenic mice were sacrificed 4.5 h after a single ip administration of LPS (100 micrograms/100 g of body wt) or vehicle. Frozen brains were mounted on a microtome and cut in 20-microns sections. mRNAs encoding CRF and GR were assayed by in situ hybridization histochemistry using 35S-labeled riboprobes, and localization of Fos-immunoreactive (Fos-ir) nuclei was determined by immunocytochemistry. Basal expression of CRF mRNA in the PVN, central nucleus of the amygdala (CeA), and geniculate complex (GN) was similar in the control and transgenic mice. LPS induced a comparable neuronal activation in the PVN of control and transgenic mice as revealed by the number of Fos-ir neurons. Moreover, the endotoxin caused a significant increase in the CRF mRNA levels within the PVN and CeA, an effect observed in both animal models. The endotoxin did not notably modulate CRF expression in other regions, such as GN. Although GR mRNA was expressed in the PVN of control mice under basal conditions, this transcript was not detected in this hypothalamic structure in LPS-treated and transgenic animals. This indicated that endogenous Type II GR mRNA is decreased in the PVN of mice expressing Type II GR antisense RNA and that gene is downregulated by LPS. Hybridization signal for CRF and GR transcripts was not notably altered by the age of mice. These results provide evidence that the basal expression of CRF and the increase of neuroendocrine CRF transcription in response to immunogenic challenges are not significantly affected by impairment of the Type II GR function. AD - Laboratory of Molecular Endocrinology, Laval University, Quebec, Canada. FAU - Laflamme, N AU - Laflamme N FAU - Barden, N AU - Barden N FAU - Rivest, S AU - Rivest S LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - UNITED STATES TA - J Mol Neurosci JT - Journal of molecular neuroscience : MN JID - 9002991 RN - 0 (Lipopolysaccharides) RN - 0 (Proto-Oncogene Proteins c-fos) RN - 0 (RNA, Antisense) RN - 0 (RNA, Messenger) RN - 0 (Receptors, Glucocorticoid) RN - 9015-71-8 (Corticotropin-Releasing Hormone) SB - IM MH - Animals MH - Brain/drug effects/metabolism MH - Corticotropin-Releasing Hormone/*genetics MH - *Gene Expression MH - Humans MH - In Situ Hybridization MH - Lipopolysaccharides/pharmacology MH - Mice MH - Mice, Transgenic MH - Paraventricular Hypothalamic Nucleus/immunology/*metabolism MH - Proto-Oncogene Proteins c-fos/genetics/metabolism MH - RNA, Antisense/*biosynthesis/metabolism MH - RNA, Messenger/metabolism MH - Rats MH - Receptors, Glucocorticoid/*genetics EDAT- 1997/06/01 00:00 MHDA- 1997/09/23 00:01 PST - ppublish SO - J Mol Neurosci. 1997 Jun;8(3):165-79. PMID- 9378611 OWN - NLM STAT- MEDLINE DA - 19971107 DCOM- 19971107 LR - 20061115 PUBM- Print IS - 0074-7742 (Print) VI - 41 DP - 1997 TI - Cerebellar output channels. PG - 61-82 AB - The cerebellum has long been regarded as involved in the control of movement, in part through its connections with the cerebral cortex. These connections were thought to combine inputs from widespread regions of the cerebral cortex and "funnel" them into the motor system at the level of the primary motor cortex. Retrograde transneuronal transport of herpes simplex virus type I has recently been used to identify areas of the cerebral cortex that are "directly" influenced by the output of the cerebellum. Results suggest that cerebellar output projects via the thalamus to multiple cortical areas, including premotor and prefrontal cortex, as well as the primary motor cortex. In addition, the projections to different cortical areas appear to originate from distinct regions of the deep cerebellar nuclei. These observations have led to the proposal that cerebellar output is composed of a number of separate "output channels." Evidence from functional imaging studies in humans and single neuron recording studies in monkeys suggests that individual output channels are concerned with different aspects of motor or cognitive behavior. AD - Veterans Administration Medical Center, Syracuse, New York, USA. FAU - Middleton, F A AU - Middleton FA FAU - Strick, P L AU - Strick PL LA - eng GR - MH11262/MH/NIMH GR - NS24328/NS/NINDS PT - Journal Article PT - Research Support, Non-U.S. Gov't PT - Research Support, U.S. Gov't, Non-P.H.S. PT - Research Support, U.S. Gov't, P.H.S. PT - Review PL - UNITED STATES TA - Int Rev Neurobiol JT - International review of neurobiology JID - 0374740 SB - IM MH - Animals MH - Brain Mapping/*methods MH - Cerebellum/*physiology MH - Humans MH - Magnetic Resonance Imaging MH - Motor Cortex/*physiology MH - Neural Pathways/physiology MH - Neurons/physiology MH - Prefrontal Cortex/physiology MH - Primates/physiology RF - 66 EDAT- 1997/01/01 00:00 MHDA- 1997/09/26 00:01 PST - ppublish SO - Int Rev Neurobiol. 1997;41:61-82. PMID- 9348568 OWN - NLM STAT- MEDLINE DA - 19971121 DCOM- 19971121 LR - 20061115 PUBM- Print IS - 0890-9091 (Print) VI - 11 IP - 9 Suppl 10 DP - 1997 Sep TI - 5-FU or UFT combined with leucovorin for previously untreated metastatic colorectal Ca. PG - 48-9 AB - This phase III study compares leucovorin plus fluorouracil (5-FU) 425 mg/m2, days 1 through 5, 28-day cycle, with oral leucovorin plus oral UFT (tegafur and uracil) 300 mg/m2, days 1 through 28, 35-day cycle, in terms of efficacy, safety, quality of life, and pharmacoeconomics. Eligible patients have not been treated previously and have measurable or evaluable metastatic colorectal cancer, an Eastern Cooperative Oncology Group performance status of 2 or less, and adequate bone marrow, liver, and renal functions. Patients are evaluated for response clinically and by computed tomography. Responses are determined by World Health Organization criteria. The study is nearing completion, with no toxicity issues requiring protocol modification. The results of this study could lead to a change to oral therapy as the standard of care for metastatic colorectal cancer, providing the efficacy and toxicity of UFT/leucovorin are at least equivalent due to the ease of administration and patient preference for oral regimens. AD - QE II Health Sciences Centre, Halifax, Nova Scotia, Canada. FAU - Skillings, J R AU - Skillings JR LA - eng PT - Clinical Trial PT - Clinical Trial, Phase III PT - Comparative Study PT - Journal Article PT - Multicenter Study PT - Randomized Controlled Trial PL - UNITED STATES TA - Oncology (Williston Park) JT - Oncology (Williston Park, N.Y.) JID - 8712059 RN - 0 (Antidotes) RN - 0 (Drug Combinations) RN - 0 (UFT(R) drug) RN - 17902-23-7 (Tegafur) RN - 51-21-8 (Fluorouracil) RN - 58-05-9 (Leucovorin) RN - 66-22-8 (Uracil) SB - IM MH - Adult MH - Antidotes/*administration & dosage MH - Antineoplastic Combined Chemotherapy Protocols/*therapeutic use MH - Colorectal Neoplasms/*drug therapy MH - Drug Combinations MH - Drug Therapy, Combination MH - Fluorouracil/administration & dosage MH - Humans MH - Leucovorin/*administration & dosage MH - Tegafur/administration & dosage MH - Uracil/administration & dosage EDAT- 1998/02/12 MHDA- 1998/02/12 00:01 PST - ppublish SO - Oncology (Williston Park). 1997 Sep;11(9 Suppl 10):48-9. PMID- 9313184 OWN - NLM STAT- MEDLINE DA - 19971029 DCOM- 19971029 LR - 20041117 PUBM- Print IS - 0884-2175 (Print) VI - 26 IP - 5 DP - 1997 Sep-Oct TI - Women's sense of well-being before and after hysterectomy. PG - 540-8 AB - OBJECTIVE: To describe women's perceived sense of well-being before and after hysterectomy by examining a broad array of outcomes experienced by women undergoing hysterectomies for benign conditions. DESIGN: Prospective, descriptive. SETTING: A regional tertiary care facility in central Texas. PARTICIPANTS: One hundred seventy-eight women presenting for hysterectomies for nononcologic reasons who completed all three periods of data collection. MAIN OUTCOME MEASURES: Subjects completed a questionnaire assessing information pertinent to their current gynecologic health and the SF-36 Health Survey before surgery and of 4 and 11 months after surgery. The women also completed the Zung Self-Rating Depression Scale preoperatively and at 4 months postoperatively. Additional demographic and medical information was extracted from the medical record. RESULTS: In the initial period after surgery, the patients experienced an improved health status. In addition, the women reported on improvement in their psychologic well-being, including less depression and improved sexual functioning. Relationships with others also improved after the surgery. CONCLUSIONS: Outcomes for these women undergoing hysterectomy for nononcologic reasons were generally positive. This information is vital for preoperative counseling by nurses of women contemplating or about to undergo this surgery. AD - Children's Health Center, Scott and White Clinic, Temple, TX 76508, USA. FAU - Lambden, M P AU - Lambden MP FAU - Bellamy, G AU - Bellamy G FAU - Ogburn-Russell, L AU - Ogburn-Russell L FAU - Preece, C K AU - Preece CK FAU - Moore, S AU - Moore S FAU - Pepin, T AU - Pepin T FAU - Croop, J AU - Croop J FAU - Culbert, G AU - Culbert G LA - eng PT - Journal Article PL - UNITED STATES TA - J Obstet Gynecol Neonatal Nurs JT - Journal of obstetric, gynecologic, and neonatal nursing : JOGNN / NAACOG JID - 8503123 SB - IM SB - N MH - Adult MH - Attitude to Health MH - Depression MH - Female MH - *Health Status MH - Humans MH - *Hysterectomy/psychology MH - Middle Aged MH - *Patient Satisfaction MH - Prospective Studies MH - Sexuality MH - Treatment Outcome MH - Uterine Diseases/*surgery EDAT- 1997/10/06 MHDA- 1997/10/06 00:01 PST - ppublish SO - J Obstet Gynecol Neonatal Nurs. 1997 Sep-Oct;26(5):540-8. PMID- 9298185 OWN - NLM STAT- MEDLINE DA - 19971117 DCOM- 19971117 LR - 20061115 PUBM- Print IS - 0003-6072 (Print) VI - 72 IP - 2 DP - 1997 Aug TI - Seasonal occurrence of yeasts and yeast-like organisms in the river Danube. PG - 77-80 AB - One hundred and seventy yeast strains belonging to 14 genera and 29 species were isolated from 112 water samples of the river Danube in the area of Bratislava. The samples were collected through the year from April to March. Saccharomyces cerevisiae, Candida maltosa, Aureobasidium pullulans, Cystofilobasidium capitatum, Rhodotorula glutinis, Geotrichum candidum, and Candida krusei were the most frequent. The basidiomycetous yeasts and yeast-like organisms with oxidative metabolism were present in approximately equal numbers to those with fermentative metabolism. Saccharomyces cerevisiae was the dominant yeast and was isolated from 50% of all samples examined and represented approximately one quarter of the yeast community. Yeast densities ranged from 100 to 21,100 CFU per litre. The highest population density was observed in October. Cryptococcus albidus, Saccharomyces cerevisiae, Rhodotorula glutinis, and Aureobasidium pullulans formed the main part of the yeast population in this month. AD - Institute of Chemistry, Slovak Academy of Sciences, Bratislava, Slovakia. FAU - Slavikova, E AU - Slavikova E FAU - Vadkertiova, R AU - Vadkertiova R LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - NETHERLANDS TA - Antonie Van Leeuwenhoek JT - Antonie van Leeuwenhoek JID - 0372625 RN - 0 (Culture Media) SB - IM MH - Aerobiosis MH - Ascomycota/*isolation & purification MH - Bacteriological Techniques MH - Basidiomycota/*isolation & purification MH - Colony Count, Microbial MH - Culture Media/metabolism MH - Ecology MH - Environmental Microbiology MH - Fermentation MH - Saccharomyces cerevisiae/isolation & purification MH - Seasons MH - Slovakia MH - *Water Microbiology EDAT- 1997/08/01 00:00 MHDA- 1997/09/23 00:01 PST - ppublish SO - Antonie Van Leeuwenhoek. 1997 Aug;72(2):77-80. PMID- 9346625 OWN - NLM STAT- MEDLINE DA - 19971114 DCOM- 19971114 LR - 20061115 PUBM- Print IS - 1074-3022 (Print) VI - 2 IP - 1 DP - 1996 Jan TI - The influence of prostaglandin E1 on platelet adherence and injury in preserved rat liver allografts. PG - 23-36 AB - We have previously shown that part of the injury sustained by cold-preserved livers on reperfusion is the consequence of platelet adhesion to sinusoidal endothelium. The purpose of the present study was to determine whether prostaglandin E1 (PGE1) can reduce the injury and if so, how to maximize this beneficial effect. Rat livers were cold-preserved in University of Wisconsin solution for 30 hours then subjected to 1-hour warm ischemia after which they were reperfused at 37 degrees C with oxygenated Krebs-Henseleit solution with or without isolated platelets. PGE1 was used to treat the donor liver during harvesting, cold preservation, and reperfusion. In some studies, PGE1 was used to pretreat platelets before exposing them to the liver, and in other studies, both liver and platelets were treated. Pretreatment of platelets with paraformaldehyde, which inactivates them, or ADP, which activates them, was also studied. Treatment of livers with PGE1 significantly decreased preservation injury when livers were reperfused in the absence of platelets. However, when platelets were added to the perfusate, prior treatment of the liver with PGE1 had relatively minor beneficial effects. Pretreatment of platelets alone with PGE1 was also beneficial, but again the effect was small. However, when both liver and platelets were treated with PGE1 there was a highly significant decrease in the extent of liver injury and platelet adhesion. Perfusate transaminase levels were lower, bile flow was improved, and histologically, livers appeared less injured. Pretreatment of platelets with paraformaldehyde produced similar results to pretreatment with PGE1. When platelets were preactivated with adenosine diphosphate, extensive hepatic injury occurred upon reperfusion despite PGE1 treatment of the liver. PGE1 can lessen preservation-reperfusion injury impressively when administered to both liver and platelets but has little effect when platelets have been preactivated. AD - Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada. FAU - Cywes, R AU - Cywes R FAU - Harvey, P R AU - Harvey PR FAU - Packham, M A AU - Packham MA FAU - Cameron, R AU - Cameron R FAU - Strasberg, S M AU - Strasberg SM LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - UNITED STATES TA - Liver Transpl Surg JT - Liver transplantation and surgery : official publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society JID - 9502504 RN - 745-65-3 (Alprostadil) SB - IM MH - Alprostadil/*pharmacology MH - Animals MH - Liver/pathology MH - *Liver Transplantation MH - Male MH - *Organ Preservation MH - Platelet Adhesiveness/*drug effects MH - Rats MH - Rats, Wistar MH - Reperfusion Injury/*prevention & control MH - Transplantation, Homologous EDAT- 1996/01/01 00:00 MHDA- 1997/11/05 00:01 AID - S1527646596000044 [pii] PST - ppublish SO - Liver Transpl Surg. 1996 Jan;2(1):23-36. PMID- 9237858 OWN - NLM STAT- MEDLINE DA - 19971124 DCOM- 19971124 LR - 20041117 PUBM- Print IS - 0014-4835 (Print) VI - 65 IP - 1 DP - 1997 Jul TI - Description and function of the ciliary nerves--some historical remarks on choroidal innervation. PG - 1-5 AB - The earliest accounts of the eye recognized its main function as providing vision, but the mechanism of how the eye functioned remained obscure for many centuries. The aim of the following work is to outline these changes in the understanding of a particular structure and function of the human body, namely, the ciliary nerves supplying the uveoscleral part of the eye. In the extensive study on the history of ophthalmology by Hirschberg (published between 1899 and 1918), the ciliary nerves and choroidal innervation are only sparsely mentioned. AD - Department of Anatomy II, Friedrich Alexander University Erlangen-Nuernberg, Erlangen, 91054, Germany. FAU - May, C A AU - May CA LA - eng PT - Historical Article PT - Journal Article PL - ENGLAND TA - Exp Eye Res JT - Experimental eye research JID - 0370707 SB - IM MH - Choroid/cytology/*injuries MH - Ciliary Body/*injuries MH - Ganglia MH - History, 16th Century MH - History, 17th Century MH - History, 18th Century MH - History, 19th Century MH - History, Ancient MH - History, Medieval MH - Humans MH - Nerve Fibers EDAT- 1997/07/01 MHDA- 1997/07/01 00:01 AID - S0014-4835(97)90312-2 [pii] AID - 10.1006/exer.1997.0312 [doi] PST - ppublish SO - Exp Eye Res. 1997 Jul;65(1):1-5. PMID- 9343384 OWN - NLM STAT- MEDLINE DA - 19971121 DCOM- 19971121 LR - 20061115 PUBM- Print IS - 0270-7306 (Print) VI - 17 IP - 11 DP - 1997 Nov TI - Elimination of defective alpha-factor pheromone receptors. PG - 6236-45 AB - This report compares trafficking routes of a plasma membrane protein that was misfolded either during its synthesis or after it had reached the cell surface. A temperature-sensitive mutant form of the yeast alpha-factor pheromone receptor (ste2-3) was found to provide a model substrate for quality control of plasma membrane proteins. We show for the first time that a misfolded membrane protein is recognized at the cell surface and rapidly removed. When the ste2-3 mutant cells were cultured continuously at 34 degrees C, the mutant receptor protein (Ste2-3p) failed to accumulate at the plasma membrane and was degraded with a half-life of 4 min, compared with a half-life of 33 min for wild-type receptor protein (Ste2p). Degradation of both Ste2-3p and Ste2p required the vacuolar proteolytic activities controlled by the PEP4 gene. At 34 degrees C, Ste2-3p comigrated with glycosylated Ste2p on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, indicating that Ste2-3p enters the secretory pathway. Degradation of Ste2-3p did not require delivery to the plasma membrane as the sec1 mutation failed to block rapid turnover. Truncation of the C-terminal cytoplasmic domain of the mutant receptors did not permit accumulation at the plasma membrane; thus, the endocytic signals contained in this domain are unnecessary for intracellular retention. In the pep4 mutant, Ste2-3p accumulated as series of high-molecular-weight species, suggesting a potential role for ubiquitin in the elimination process. When ste2-3 mutant cells were cultured continuously at 22 degrees C, Ste2-3p accumulated in the plasma membrane. When the 22 degrees C culture was shifted to 34 degrees C, Ste2-3p was removed from the plasma membrane and degraded by a PEP4-dependent mechanism with a 24-min half-life; the wild-type Ste2p displayed a 72-min half-life. Thus, structural defects in Ste2-3p synthesized at 34 degrees C are recognized in transit to the plasma membrane, leading to rapid degradation, and Ste2-3p that is preassembled at the plasma membrane is also removed and degraded following a shift to 34 degrees C. AD - Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester 01655-0122, USA. jennessd@ummed.edu FAU - Jenness, D D AU - Jenness DD FAU - Li, Y AU - Li Y FAU - Tipper, C AU - Tipper C FAU - Spatrick, P AU - Spatrick P LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - UNITED STATES TA - Mol Cell Biol JT - Molecular and cellular biology JID - 8109087 RN - 0 (Fungal Proteins) RN - 0 (Receptors, Mating Factor) RN - 0 (Receptors, Peptide) RN - 0 (Transcription Factors) SB - IM MH - Biological Transport MH - Cell Compartmentation MH - Cell Membrane/metabolism MH - Cloning, Molecular MH - Fungal Proteins/chemistry/genetics/*metabolism MH - Models, Molecular MH - Mutation MH - Protein Conformation MH - Protein Folding MH - Receptors, Mating Factor MH - Receptors, Peptide/chemistry/genetics/*metabolism MH - Reproduction MH - Saccharomyces cerevisiae/metabolism MH - Sequence Analysis, DNA MH - *Transcription Factors MH - Vacuoles/metabolism EDAT- 1997/10/29 MHDA- 1997/10/29 00:01 PST - ppublish SO - Mol Cell Biol. 1997 Nov;17(11):6236-45. PMID- 9259380 OWN - NLM STAT- MEDLINE DA - 19971106 DCOM- 19971106 LR - 20061115 PUBM- Print IS - 0148-7299 (Print) VI - 74 IP - 4 DP - 1997 Jul 25 TI - Obsessive compulsive symptoms in Gilles de la Tourette syndrome and obsessive compulsive disorder: differences by diagnosis and family history. PG - 432-8 AB - The distribution of obsessive compulsive symptoms was compared in 16 individuals with primary obsessive compulsive disorder (OCD) and 16 individuals with Gilles de la Tourette syndrome (GTS) and associated obsessive compulsive behaviors (OCB). The two groups showed significant differences in the distribution of OC symptomatology. Furthermore, those OCD probands who shared a similar symptom profile with GTS individuals all had a positive family history of OCD. All of the other OCD probands were isolated cases. Implications of this finding on the etiology and pathogenesis of the two disorders are discussed. AD - Department of Psychiatry, University of London, United Kingdom. FAU - Eapen, V AU - Eapen V FAU - Robertson, M M AU - Robertson MM FAU - Alsobrook, J P 2nd AU - Alsobrook JP 2nd FAU - Pauls, D L AU - Pauls DL LA - eng GR - MH-00508/MH/NIMH PT - Comparative Study PT - Journal Article PT - Research Support, U.S. Gov't, P.H.S. PL - UNITED STATES TA - Am J Med Genet JT - American journal of medical genetics JID - 7708900 RN - 50-67-9 (Serotonin) RN - 51-61-6 (Dopamine) SB - IM MH - Adolescent MH - Adult MH - Basal Ganglia/physiopathology MH - Child MH - *Compulsive Behavior MH - Dopamine/physiology MH - Female MH - Frontal Lobe/physiopathology MH - Gyrus Cinguli/physiopathology MH - Humans MH - Male MH - Middle Aged MH - *Obsessive Behavior MH - Obsessive-Compulsive Disorder/etiology/genetics/physiopathology/*psychology MH - Psychological Tests MH - Serotonin/physiology MH - Thalamus/physiopathology MH - Tourette Syndrome/etiology/genetics/physiopathology/*psychology EDAT- 1997/07/25 MHDA- 2000/06/20 09:00 AID - 10.1002/(SICI)1096-8628(19970725)74:4<432::AID-AJMG15>3.0.CO;2-J [pii] PST - ppublish SO - Am J Med Genet. 1997 Jul 25;74(4):432-8. PMID- 9379007 OWN - NLM STAT- MEDLINE DA - 19971112 DCOM- 19971112 LR - 20061115 PUBM- Print IS - 0022-1767 (Print) VI - 159 IP - 9 DP - 1997 Nov 1 TI - Regulation of C-C chemokine production by murine T cells by CD28/B7 costimulation. PG - 4150-3 AB - C-C chemokines play an important role in recruitment of T lymphocytes to inflammatory sites. T lymphocytes secrete chemokines, but the activation requirements for chemokine production by T cells are uncertain. We studied the regulation of C-C chemokine production by CD28 costimulatory signals by murine T lymphocytes. Splenocytes from BALB/c mice cultured with